10x Chromium System

10x Genomics' Chromium technology partitions reactions into nanoliter-scale droplets containing uniquely barcoded beads called GEMs (Gel Bead-In EMulsions). This core technology can be used to partition single cells or high molecular DNA to prepare next generation sequencing libraries in parallel . We provide training and run samples as a service on the Chromium system. Please contact Claire Reardon to discuss your experimental plans. For more information about the technology, please visit 10xGenomics

Single-cell Workflow

The single-cell assay partitions individual cells into GEMs that uniquely barcode hundreds to thousands of cells with a capture efficiency of 65%. 3’ end counting is used to interrogate gene expression and characterize cells of a heterogeneous population. For more information about the assay, please visit 10xGenomics.Single-cell v2 protocol

Genome Assay Workflow

The genome assay partitions high molecular weight (HMW) DNA into individual GEMs to capture long-range information using Illumina next-generation sequencing. The assay requires low input of high molecular weight DNA and produces "linked read" information which can be used to assemble genomes, assess structural variants, and phase across haplotype blocks >10 Mb.Please contact Claire Reardon to discuss your experimental plans. For more information about the assay, please visit 10xGenomics

Genome Workflow