Single Cell 3' and 5' Workflow
10x single-cell 3' and 5' assays partition individual cells into GEMs that uniquely barcode hundreds to thousands of cells with a capture efficiency of 65%. 3’ or 5' end counting determines gene expression and characterizes cells of a heterogeneous population. In addition to expression profiling, the 5' assay enables immune profiling by enriching barcoded cDNA for V(D)J sequences of T or B cells. Both the 3' and 5' assays can be combined with "Feature Barcoding" technology which determines expression of cell-surface proteins through oligo-labelled antibodies. For more information about these assays, please visit 10xGenomics.
Single Cell ATAC Workflow
The single cell ATAC assay allows for determination of the accessibility of chromatin on a single cell level. A sample of hundreds to thousands of nuclei undergoes a transposition reaction and the transposed nuclei are then partitioned into GEMs which uniquely barcode accessible DNA fragments. For more information about this assay, please visit 10xGenomics.