10x Chromium System

10x Genomics' Chromium technology partitions reactions into nanoliter-scale droplets containing uniquely barcoded beads called GEMs (Gel Bead-In EMulsions). This core technology can be used to partition single cells, nuclei, or high molecular weight gDNA to prepare next generation sequencing libraries in parallel. We provide training and run samples as a service on the Chromium system. Please contact Claire Reardon to discuss your experimental plans. For more information about the technology, please visit 10xGenomics

Single Cell 3' and 5' Workflow

10x single-cell 3' and 5' assays partition individual cells into GEMs that uniquely barcode hundreds to thousands of cells with a capture efficiency of 65%. 3’ or 5' end counting determines gene expression and characterizes cells of a heterogeneous population. In addition to expression profiling, the 5' assay enables immune profiling by enriching barcoded cDNA for V(D)J sequences of T or B cells. Both the 3' and 5' assays can be combined with "Feature Barcoding" technology which determines expression of cell-surface proteins through oligo-labelled antibodies. For more information about these assays, please visit 10xGenomics.Single-cell v2 protocol

Single Cell ATAC Workflow

The single cell ATAC assay allows for determination of the accessibility of chromatin on a single cell level. A sample of hundreds to thousands of nuclei undergoes a transposition reaction and the transposed nuclei are then partitioned into GEMs which uniquely barcode accessible DNA fragments. For more information about this assay, please visit 10xGenomics.

ATAC workflow