Rates below effective July 1st, 2018 - June 30th 2019. Please review our billing policy for information on how to submit billing information, charge codes and purchase orders.
Fees
Flow Cytometry
Usage Fees
|
Instrument |
Harvard Account Code |
PO Payment |
|
Staff-Run Sorting |
$115/hr |
$161/hr |
|
Self-Run Analysis |
$20/hr |
$27/hr |
|
Self-Run Sorting (Aria, Biosorter, S3) |
$53/hr |
$74 (Biosorter/S3 only) |
|
Self-Run Multisizer |
$20/hr |
$27/hr |
Training Fees
|
Instrument |
Harvard Account Code |
PO Payment |
|
Analyzer (LSRII or Fortessa) (prerequisite: Intro to Flow Workshop) |
$141 |
$198 |
|
HTS autosampler (prerequisite: Analyzer Training) |
$133 |
$186 |
|
FACSAria (prerequisite: Analyzer Training) |
$700 |
not available |
|
Biosorter |
$700 |
$980 |
|
BioRad S3 |
$319 |
$447 |
|
Multisizer |
$141 |
$198 |
Sequencing
Illumina Fees FY19
For help choosing the right type of sequencing, see our experimental design advice our decision tree and our instrument capabilities. Request a consultation or inquire about pricing for custom read lengths by emailing illumina_submission-list@lists.fas.harvard.edu.
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Single Lane |
Full Flow Cell (one sample over all lanes) |
||
|
Harvard Account Code Payment |
PO Payment |
Harvard Account Code Payment |
PO Payment |
|
|
HiSeq High Output v4 1 x 50 bp
|
$976 |
$1,366 |
- |
- |
|
HiSeq High Output v4 2 x 125 bp
|
$2,481 |
$3,473 |
- |
- |
|
HiSeq Rapid 1 x 50 bp
|
$1,273 |
$1,782 |
$2,052 |
$2,872 |
|
HiSeq Rapid 2 x 75 bp
|
$1,772 |
$2,481 |
$3,050 |
$4,270 |
|
HiSeq Rapid 2 x 150 bp
|
$2,556 |
$3,579 |
$4,619 |
$6,467 |
|
HiSeq Rapid 2 x 250 bp
|
$3,341 |
$4,677 |
$6,188 |
$8,663 |
|
NextSeq Mid - up to 166 cycles
|
- |
- |
$1,409 |
$1,972 |
|
NextSeq Mid - up to 316 cycles
|
- |
- |
$2,266 |
$3,172 |
|
NextSeq High - up to 92 cycles
|
- |
- |
$1,817 |
$2,544 |
|
NextSeq High - up to 166 cycles
|
- |
- |
$3,192 |
$4,469 |
|
NextSeq High - up to 316 cycles
|
- |
- |
$5,113 |
$7,159 |
| NovaSeq SP 100 cycles | $1,688 | $2,363 | $2,999 | $4,199 |
| NovaSeq SP 300 cycles | $2,808 | $3,932 | $5,240 | $7,336 |
| NovaSeq SP 500 cycles | $3,616 | $5,062 | $6,855 | $9,597 |
| NovaSeq S1 100 cycles | $2,567 | $3,593 | $4,757 | $6,659 |
| NovaSeq S1 200 cycles | $3,232 | $4,524 | $6,087 | $8,521 |
| NovaSeq S1 300 cycles | $4,138 | $5,794 | $7,900 | $11,060 |
| NovaSeq S2 100 cycles | $3,469 | $4,857 | $6,562 | $9,186 |
| NovaSeq S2 200 cycles | $6,469 | $9,057 | $12,562 | $17,586 |
| NovaSeq S2 300 cycles | $6,087 | $8,522 | $11,797 | $16,516 |
| NovaSeq S4 200 or 300 cycles | inquire | inquire | inquire | inquire |
Library Preparation Fees
|
Full-Service High Throughput Library Preparation Price/sample, QC extra (see add-ons below) |
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| Harvard Code Payment | PO Payment | |||
| Rush | Queue | Rush | Queue | |
|
Kapa DNA HyperPlus 1/4 volume |
$41 |
$16 |
$57 |
$23 |
|
Kapa mRNA Hyperprep (incl. polyA selection) |
$69 |
$48 |
$97 |
$67 |
| Kapa RNA Hyperprep with RiboErase | $111 | $92 | $155 | $129 |
| Nextera 1/4 volume | $23 | $16 | $32 | $22 |
| SmartSeq v4 + Nextera 1/4 volume | $46 | $35 | $65 | $50 |
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Full-Service QC Add-ons - price per sample or per pool |
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| Harvard Code Payment | PO Payment | |||
| Qubit and Nanodrop (up to 24 samples) | $75 | $106 | ||
| Quantify and Normalize up to 96 samples | $211 | $295 | ||
| Agilent TapeStation QC check | $15 | $21 | ||
| Agilent Bioanalyzer QC check | $29 | $41 | ||
| QPCR Library Quantification | $39 | $55 | ||
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Additional Services - price per sample or per pool |
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| Harvard Code Payment | PO Payment | |||
| Covaris shearing | $36 | $51 | ||
| Pippin Prep/Blue Pippin size selection | $73 | $102 | ||
| MagBead Cleanup | $31 | $44 | ||
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Independent (Self-Run) Use of Apollo 324 |
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| Harvard Code Payment | PO Payment | |
|
Apollo 324 training fee |
$248 | $347 |
|
Self-Run Apollo use (includes plastics) |
$10/sample | $14/sample |
|
High Throughput Self-Run Apollo use (email core for 48- or 32-sample script price) |
$5/sample | $7/sample |
|
Reagents |
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| Harvard Code Payment | PO Payment | |
|
PCRClean DX Magnetic Beads (Aline), 1 ml |
$6 |
n/a |
|
Indexed primer aliquot for RNA library |
$3 |
$4 |
|
Unique Dual Indexed Adapters, 96 @ 15uM |
$937 |
n/a |
|
Unique Dual Indexed Adapters, 96 @ 1.5uM |
$125 |
n/a |
Single Cell
|
|
Harvard Account Code Payment |
PO Payment |
|
10x Chromium |
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Full service Single Cell 3' v2 library prep |
$2088/sample |
$2924/sample |
|
Full service Genome Assay library prep |
$1249/sample |
$1749/sample |
|
10x GEM formation training - with reagents (required for independent use) |
$374/attendee |
$524/attendee |
|
10x full workflow training, any assay |
inquire |
inquire |
|
10x Chromium independent use, any assay |
$73/chip |
$102/chip |
|
SmartSeq v4 - see costs on Flow Cytometry and Library Prep Tabs |
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Fluidigm C1/Biomark |
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|
C1 Training |
$410 |
$574 |
|
C1 Independent Use |
$100/chip |
$140/chip |
|
C1 Full-Service |
inquire |
inquire |
|
Biomark Training |
$252 |
$353 |
|
Biomark Independent Use |
$100/chip |
$140/chip |
Other Fees
Training Fees
|
Instrument Training Fee |
Cost - Harvard Account Code |
Cost - PO Payment |
|
Agilent BioAnalyzer (reagents extra) |
$248 |
$347 |
|
Agilent TapeStation (reagents extra) |
$124 |
$174 |
|
Autogen |
$289 |
$304 |
|
Biomek |
$248 |
$347 |
|
Covaris |
$93 |
$130 |
| Mantis | $93 | $130 |
|
Pippin Prep/Blue Pippin |
$93 |
$130 |
|
QX200 ddPCR |
$347 |
$486 |
|
Additional Attendee at QX200 Training |
$37 |
$52 |
|
Real Time PCR (ABI7900, BioRad CFX, Biomark) |
$252 |
$353 |
|
Typhoon |
$101 |
$141 |
Usage Fees
|
Instrument Usage Fee |
Cost - Harvard Account Code |
Cost - PO Payment |
|
Agilent Bioanalyzer self-run |
$50/chip |
$70/chip |
| Agilent TapeStation D1000/D5000 tape + use fee | $43/tape | $60/tape |
|
Agilent TapeStation HSD1000/HSD5000 tape + use fee |
$61/tape |
$86/tape |
|
Agilent TapeStation gDNA tape + use fee |
$63/tape |
$89/tape |
|
Agilent TapeStation RNA tape + use fee |
$40/tape |
$55/tape |
|
Agilent TapeStation HSRNA tape + use fee |
$45/tape |
$63/tape |
|
Autogen |
$8/hr |
$11/hr |
|
Biacore 3000 |
$56/hr |
$79/hr |
|
Fluidigm Biomark |
$100/chip |
$140/chip |
|
Covaris (includes tube) |
$5/sample |
$6/sample |
| Mantis |
$4/hr |
$6/hr |
| Pippin Prep/Blue Pippin |
$8/hr |
$12/hr |
| Plate Readers: Bioscreen, Epoch |
$1/hr |
$2/hr |
| Plate Readers: FlexStation 3, Spectramax L | $20/hr | $28/hr |
| Plate Readers: Neo2 | $4/hr | $6/hr |
| Plate Readers: Spectramax i3 | $6/hr | $8/hr |
| Plate Readers: Safire 2 | $14/hr | $18/hr |
|
QX200 Droplet Generator |
$19/ 8-sample cartridge |
$26/ 8-sample cartridge |
|
QX200 Droplet Reader |
$11/ 8 samples |
$15/ 8 samples |
|
Real Time PCR (ABI7900, BioRad CFX Touch) |
$4/hr |
n/a |
|
Typhoon Imager |
$32/hr |
$44/hr |