#  FY27 Long Read Library Prep & Sequencing 

 



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##  PacBio Revio Library Prep & Sequencing 

SortPacBio Revio Sequencing

REVIO SMRT Cell ~ 90Gb

Harvard

Account Code

PO

Payment

SMRT Cell 25M sequencing, per cell



$1,564



$2,190



SMRT Cell Nx sequencing, per cell - 3 acquisitions



$2,578



$3,609







SortPacBio Library Preparation

(per sample, does not include sequencing)

SMRTbell 3.0 Library Prep

 Harvard Account Code

 PO Payment

 Whole Genome

(optional barcodes included)



$702



$982



AmpliFi



$806



$1,128



IsoSeq v2

(for Kinnex)



$363



$508



Amplification for Kinnex

(per plate)



$1,316



$1,842



Kinnex



$1,543



$2,160









 

##  Oxford Nanopore Library Prep & Sequencing 

SortOxford Nanopore Technologies (ONT) Sequencing

PromethION ~ 80-90 Gb

~80-90x coverage for 1 Gb Genome

MinION ~10 Gb

~1x coverage for 1 Gb Genome

Per Flow Cell

Harvard

Account Code

PO

Payment

Per Flow Cell

Harvard

Account Code

PO

Payment

PromethION



$1,263



$1,769





MinION



$913



$1,279



Nuclease Flush & Reload



$157



$220





Nuclease Flush & Reload



$157



$220







SortOxford Nanopore Library Prep

(per sample, does not include sequencing)

ONT Library Prep Kit

 Harvard Account Code

 PO Payment

 Ligation

(LSK114)



$428



$599



Ligation with Native Barcodes

(SQK-NBD114.24)



$336



$471



Rapid

(SQK-RAD004)



$164



$229



Barcoded Rapid

(SQK-RBK114.24)



$112



$157



Direct RNA

(RNA-004)



$730



$1,022



PCR cDNA

(SQK-PCS114)



$882



$1,235



Barcoded PCR cDNA

(SQK-PCB114.24)



$389



$544



Direct cDNA

(LSK-114)



$550



$770



Barcoded Direct cDNA

(NBD114.24)



$331



$463



10x Genomics

(LSK114)



$901



$1,262



10x Genomics Visium 3' HD

(LSK114)



$922



$1,291