#  FY27 NGS Sequencing 

 



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##  NovaSeq X Plus 

 

###  25B Flow Cell | 8 Lanes Per Flow Cell 

SortPrices Per Lane

2.5 Billion Reads

Read

Length

Harvard

Account

Code

PO

Payment

**\*100 cycle**



$1,403



$1,964



**\*200 cycle**



$2,143



$3,001



**300 cycle**



$2,426



$3,396







\*infrequently run, expect a long wait for single lanes



 

###  10B Flow Cell | 8 Lanes Per Flow Cell 

SortPrices Per Lane

1 Billion Reads

Read

Length

Harvard

Account

Code

PO

Payment

**\*100 cycle**



$1,030



$1,442



**\*200 cycle**



$1,193



$1,671



**300 cycle**



$1,314



$1,840







\*infrequently run, expect a long wait for single lanes



 

###  5B Flow Cell | 8 Lanes Per Flow Cell 

SortPrices Per Lane

562 Million Reads

Read

Length

Harvard

Account

Code

PO

Payment

**\*100 cycle**



$616



$863



**\*200 cycle**



$724



$1,013



**300 cycle**



$799



$1,119







\*infrequently run, expect a long wait for single lanes



 

###  1.5B Flow Cell | 2 Lanes Per Flow Cell 

SortPrices Per Lane

600 Million Reads

Read

Length

Harvard

Account

Code

PO

Payment

**\*100 cycle**



$1,679



$2,351



**\*200 cycle**



$1,929



$2,700



**\*300 cycle**



$2,028



$2,840







\*infrequently run, expect a long wait for single lanes



 

##  NextSeq 1000 

SortP1 Flow Cell

P2 Flow Cell

90 Million Reads

360 Million Reads

Read

Length

Harvard

Account

Code

PO

Payment

Read

Length

Harvard

Account

Code

PO

Payment

**100 cycle**



$1,174



$1,644





**100 cycle**



$1,581



$2,214



**300 cycle**



$1,480



$2,072



**200 cycle**



$1,759



$2,463



**600 cycle**



$2,282



$3,194



**300 cycle**



$1,876



$2,626







**600 cycle**



$3,861



$5,406









 

##  MiSeq 

 

###  Nano &amp; Micro Flow Cells 

SortNano v2 Flow Cell

Micro v2 Flow Cell

1 Million Reads

4 Million Reads

Read

Length

Harvard

Account

Code

PO

Payment

Read

Length

Harvard

Account

Code

PO

Payment

**300 cycle**



$685



$959





**300 cycle**



$893



$1,251



**500 cycle**



$766



$1,073











 

###  v2 &amp; v3 Flow Cells 

Sortv2 Flow Cell

v3 Flow Cell

15 Million Reads

25 Million Reads

Read

Length

Harvard

Account

Code

PO

Payment

Read

Length

Harvard

Account

Code

PO

Payment

**50 cycle**



$1,421



$1,990





**150 cycle**



$1,547



$2,166



**300 cycle**



$1,743



$2,440



**600 cycle**



$2,676



$3,746



**500 cycle**



$1,925



$2,695











 

##  NextSeq 500 

[Inquire - email the team ](mailto:<illumina_submission-list@lists.fas.harvard.edu>)