Illumina Instruments

Before beginning a project, please email illumina_submission-list@lists.fas.harvard.edu to discuss your experiment. 

nextseq500

Illumina NextSeq 500

  • Single Read or Paired End reads up to 150 bp long
  • Cycles availabe (used for sequence and index reads):
    • 75 cycle kit: up to 92 bases
    • 150 cycle kit: up to 166 bases
    • 300 cycle kit: up to 316 bases
  • Two Flow Cell Types:
    • High ~ 400 million clusters*
    • Mid ~ 130 million clusters*
highseq

Illumina HiSeq 2500

  • Single Read or Paired End reads up to 250 bp long
  • Two flow cell types:
    • High Output (Standard) v4 - 8 lanes, up to 250 million clusters each*
    • Rapid Run - 2 lanes, up to 150 million clusters each*
  * When Bauer Core performs sample quantification, we guarantee to deliver at least 2/3 of the advertised optimal number of clusters
   

Ancillary Equipment for Sequencing Projects

The core offers instruments for sample shearing, size selection, library QC and quantification.

apollo324

Apollo 324

This instrument automates next-generation sequencing library preparation.

  • Reagent kits are available for DNA sequencing, RNA-Seq or ChIP-seq.
  • Compatible with indexing/multiplexing.
  • Prepare 1-48 samples at a time.

For more information on the Apollo 324, visit http://www.wafergen.com/apollo-324/.

 
pippin-prep

Pippin Prep and Blue Pippin

These instruments automate nucleic acid size selection using gel cassettes.

  • Choose broad or tight size range cuts.
  • Choose the % gel that will allow for selection of your desired size.
  • Prepare 4 samples at a time along with a reference.

For more information, visit http://www.sagescience.com/.

eGel

Life Tech E-gel system

Together the E-gel iBase and Safe Imager automate nucleic acid electrophoresis and size selection using pre-cast gels. Features include fast run times, programmable control, and real time monitoring of DNA migration. Researchers must supply their own gels for use on the system.

For more information, visit http://www.lifetechnologies.com.