Instruments

Before beginning a project, please email illumina_submission-list@lists.fas.harvard.edu to discuss your experiment. 

NovaSeq

Illumina NovaSeq

  • Single Read or Paired End reads up to 250 bp long
  • Four Flow Cell Types:
    • SP: 2 lanes, ~ 400 million clusters/lane*
    • S1: 2 lanes, ~ 800 million clusters/lane*
    • S2: 2 lanes, ~ 2 billion clusters/lane*
    • S4: 4 lanes, ~ 2.5 billion clusters/lane*
  • Kits availabe (used for sequence and index reads):
    • 100 cycle kit: up to 130 bases
    • 200 cycle kit (not available on SP): up to 230 bases
    • 300 cycle kit: up to 330 bases
    • 500 cycle kit (SP flowcell only): up to 516 bases
    nextseq500

    Illumina NextSeq 500

    • Single Read or Paired End reads up to 150 bp long
    • Two Flow Cell Types:
      • High ~ 400 million clusters*
      • Mid ~ 130 million clusters*
    • Kits availabe (used for sequence and index reads):
      • 75 cycle kit: up to 92 bases
      • 150 cycle kit: up to 166 bases
      • 300 cycle kit: up to 316 bases
      highseq

      Illumina HiSeq 2500

      • Single Read or Paired End reads up to 250 bp long
      • Two flow cell types:
        • High Output (Standard) v4 - 8 lanes, up to 250 million clusters/lane*
        • Rapid Run - 2 lanes, up to 150 million clusters/lane*
        * When Bauer Core performs sample quantification, we guarantee to deliver at least 2/3 of the advertised optimal number of clusters
      MinIon

      Oxford Nanopore MinION

      Generate 10-30 Gb of sequence data per flowcell with reads lengths up to hundreds of kb. We offer limited support for this sequencing technology, so please contact Claire Hartmann (claire@cgr.harvard.edu) to discuss your needs

      Ancillary Equipment for Sequencing Projects

      The core offers instruments for sample shearing, size selection, library QC and quantification.

      apollo324

      Apollo 324

      This instrument automates next-generation sequencing library preparation.

      • Reagent kits are available for DNA sequencing, RNA-Seq or ChIP-seq.
      • Compatible with indexing/multiplexing.
      • Prepare 1-48 samples at a time.

      For more information on the Apollo 324, visit http://www.wafergen.com/apollo-324/.

       
      pippin-prep

      Pippin Prep and Blue Pippin

      These instruments automate nucleic acid size selection using gel cassettes.

      • Choose broad or tight size range cuts.
      • Choose the % gel that will allow for selection of your desired size.
      • Prepare 4 samples at a time along with a reference.

      For more information, visit http://www.sagescience.com/.

      eGel

      Life Tech E-gel system

      Together the E-gel iBase and Safe Imager automate nucleic acid electrophoresis and size selection using pre-cast gels. Features include fast run times, programmable control, and real time monitoring of DNA migration. Researchers must supply their own gels for use on the system.

      For more information, visit http://www.lifetechnologies.com.