# Instruments at the Bauer Core ## expand\_more To learn about any of these instruments, please contact the [Core Staff](mailto:illumina_submission-list@lists.fas.harvard.edu). [To see our full list of instrumentation see here. ](/file_url/178) [See our Resources page for additional protocols and documentation. ](/resources) Sample Analysis and QC Real-Time qPCR Plate Readers Other Instruments at the Bauer Core ## Sample Analysis and QC ## Fragment Analysis and Quantification **Agilent TapeStation 4200** ![1600x1600_4200TS_image2](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/2025-02/1600x1600_4200TS_image2.jpg?itok=Fk57wOxJ) The TapeStation rapidly analyzes the size and quantity of RNA and DNA samples. Tapes allow for small-scale gel electrophoresis using very little sample. Each tape can analyze up to 16 samples which may be run all together or at different times over two weeks. For more information, see [Agilent's website.](https://www.agilent.com/en/product/automated-electrophoresis/tapestation-systems/tapestation-instruments/4200-tapestation-system-228263) **Agilent Femto Pulse** ![Agilent Femto Pulse](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/femtopulse.jpg?itok=VvwRgtzX) The Femto Pulse analyzes samples using pulsed-field capillary electrophoresis. It is ideal for analyzing High Molecular Weight gDNA and low-concentration RNA samples. For more information, see [Agilent's website.](https://www.agilent.com/en/product/automated-electrophoresis/femto-pulse-systems/femto-pulse-system/femto-pulse-system-365750) **Agilent Bioanalyzer 2100** ![Agilent 2100 Bioanalyzer](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/agilent_2100_bioanalyzer.jpg?itok=Ev0e7DrE) The Bioanalyzer uses microfluidic technology to perform automated quantification and sizing to analyze RNA, DNA, and proteins.. For more information, see [Agilent's website.](https://www.agilent.com/en/product/automated-electrophoresis/bioanalyzer-systems/bioanalyzer-instrument/2100-bioanalyzer-instrument-228250) **Qubit Fluorometer** ![Qubit top with main menu screen_650x600](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/2025-02/Qubit%20top%20with%20main%20menu%20screen_650x600.jpg-650.jpg?itok=B8jy8abh) The Qubit enables precise quantification of dsDNA, ssDNA, RNA, and protein using a fluorescent dye. For more information, see [Thermo Fisher's website.](https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/models/qubit-4.html) ## Spectrophotometers- Sample Purity **NanoDrop ND-1000 Spectrophotometer** ![71sHcA4hueL__12818.1570127233](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/2025-02/71sHcA4hueL__12818.1570127233-852x800.jpg?itok=a5GDnccJ) The Nanodrop is a UV-Visible Spectrophotometer designed to read the absorbance of 1-2 µl samples without a cuvette. Most samples can be run without dilution. It has a wavelength range of 220-750nm. For more information, see [Thermo Fisher's website.](http://www.nanodrop.com/) **Denovix DS-11** ![DS-11 Spectrophotometer](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/ds-11.jpg?itok=6jiH2d8I) The DS-11 is a UV-Visible Spectrophotometer capable of measuring the absorbance of samples using only 1 µl. It has a wavelength range of 190-840nm. For more information, see [Denovix's website.](https://www.denovix.com/products/ds-11-fx-spectrophotometer-fluorometer/) ## Automated Size Selection Blue Pippin & Pippin Prep ![Sage Sciences Blue Pippin & Pippin Prep Instruments](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/pippins.jpg?itok=NGlxc7RC) Sage Science's pippin instruments perform automated size selection for many sample types, such as High Molecular Weight gDNA and tight library size selection. For more information, [see Sage Science's website](https://sagescience.com/). ## Real-Time qPCR ## Droplet Digital PCR (ddPCR) **Bio-Rad QX200 Droplet Generator & Reader** ![Bio-Rad QX200 Droplet Digital PCR Instruments](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/qx200.jpg?itok=AXR9yaOK) - Rare event detection - Copy Number Variation (especially at high copy number) - Gene expression - Low expression - Small fold changes For more information, see [Bio-Rad's website.](https://www.bio-rad.com/en-us/life-science/digital-pcr/qx200-droplet-digital-pcr-system) **QIAcuity Digital PCR System - Demo System** ![Qiagen Qiacuity](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/2025-05/Qiagen%20Qiacuity.jpg?itok=pDqYqckp) - [ Details here](https://www.qiagen.com/us/products/instruments-and-automation/pcr-instruments/qiacuity-digital-pcr-system?cmpid=CM_PCR_dPCR_Traffic_0123_SEA_GA_NA&s_kwcid=AL!16953!3!581077163254!p!!g!!qiagen%20qiacuity&gad_source=1&gad_campaignid=16142545878&gbraid=0AAAAAD-RrCzHgwOsFlLABpau9SW4TM8PH&gclid=Cj0KCQjwoZbBBhDCARIsAOqMEZV7Fsh4mxP5hWsQi32J1ZlE4mWYpILG-EXPvcQXHgg63gEwCdgTu6AaAgyoEALw_wcB) ## Real-Time Quantitative PCR **Bio-Rad CFX96 & 384** ![Bio-Rad CFX96 RTqPCR](/sites/g/files/omnuum7141/files/styles/hwp_1_1__360x360_scale/public/bsctestsite/files/cfx96.jpg?itok=QTHRuqq8) - qPCR - Gene detection & quantification - Primer specificity and optimization [CFXTouch RT-PCR Protocol](/file_url/148) For more information, see [Bio-Rad's website](https://www.bio-rad.com/en-us/product/cfx96-touch-real-time-pcr-detection-system?ID=LJB1YU15). **Bio-Rad Thermalcyclers C1000 Touch and S1000** ![Bio-Rad Thermalcycler](/sites/g/files/omnuum7141/files/styles/hwp_1_1__360x360_scale/public/bsctestsite/files/thermalcycler.jpg?itok=aay6lmK9) - PCR amplification - Incubations For more information, see [Bio-Rad's website](https://www.bio-rad.com/en-us/category/thermal-cyclers-for-pcr?ID=75f1b406-3746-4580-a998-74245b094f56). ## Plate Readers **BioTek Synergy Neo2** ![BioTek Neo2](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/biotekneo2.jpg?itok=ik83WEsI) [See here for more information.](/file_url/126) **Epoch 2** ![BioTek Epoch2](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/epoch2.jpg?itok=lheHom--) [See here for more information.](https://www.biotek.com/products/detection-microplate-readers/epoch-2-microplate-spectrophotometer/) **FlexStation III** ![FlexStation](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/flexstation.jpg?itok=OgIbVV8S) [See here for more information.](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/flexstation-3-reader) **SpectraMax i3** ![SpectraMax i3](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/spectramaxi3.jpg?itok=GK89KIVE) [See here for more information.](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-i3x-readers) ## Other Instruments at the Bauer Core ## Nucleic Acid Shearing **Covaris S220** ![Covaris S220 Instrument](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/covariss220.jpg?itok=pYeLZkAQ) The Covaris S220 system's controlled, reproducible DNA shearing method generates fragments with tight size distributions centered from 100 to 5000 bp. To request Covaris shearing service, please email this [form](https://drive.google.com/file/d/1uiCDqxxJVcwc3Jpel7xdcpOgvTtzBcjI/view?usp=sharing) to [Core Staff.](mailto:illumina_submission-list@lists.fas.harvard.edu) For more information, see [Covaris' website.](https://www.covaris.com/s220-focused-ultrasonicator-500217) - [Using the Covaris S220 Protocol](/file_url/151) - [Covaris Shearing Protocol](/file_url/152) **Fisher 550 Sonicator** ![Sonicator](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/fisher550.jpg?itok=PMlz6XK8) Used for DNA fragmentation and cell disruption. For more information, see [Fisher Scientific's website.](https://www.fishersci.com/shop/products/sonifier-sfx250-sfx550-cell-disruptors/p-6713003) - [Fisher 550 Sonicator Protocol](/file_url/153) **Diagenode Megaruptor 3** ![Megaruptor3](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/megaruptor3.jpg?itok=jpFENtlE) For more information, see[ Diagenode's website](https://www.diagenode.com/en/p/megaruptor-3#). ## Tissue Dissociation and Homogenization **GentleMACS Octo Dissociator** ![GentleMACS Octo Dissociator](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/octomacs.jpg?itok=93UptkI5) The GentleMACS is a benchtop instrument for automated tissue dissociation for single-cell suspensions or tissue homogenization for RNA isolation of up to eight samples at a time. The instrument uses specially designed disposable tubes that depend on your application. Typical samples used on the instrument include human or mouse tumors, neural tissues, and heart or brain samples, and it can also handle plant samples for nuclei isolation. Researchers can be trained to use the instrument independently. To learn about using this instrument, please [Core Staff.]() For more information, see [Miltenyi's website.](https://www.miltenyibiotec.com/US-en/products/gentlemacs-octo-dissociator-with-heaters.html) ## Imaging Systems **Azure Sapphire Imager** ![Sapphire Imager](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/azure_sapphire.jpg?itok=y13ZEoGL) This multimode imager acquires fluorescent, chemiluminescent, and phosphor images with a resolution down to 10 microns. Fluorescent detection uses 4 lasers/filters: ex 488/em 518 bp22, ex 520/em 565 bp24, ex 658/em 710 bp40, ex 785/em 832 bp37. RGB fluorescent signal is captured using a PMT, while NIR is captured with an avalanche photodiode. Chemiluminescent and visible images are captured with a high-resolution CCD camera. For more information, see [Azure's website.](https://azurebiosystems.com/sapphire/) **Azure c200 Imager** ![C200](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/c200.jpg?itok=E5lb50Ch) This gel imager uses UV for Ethidium bromide-stained DNA gels, blue light for SYBRSafe or similar dyes, and white light for Silver stain or Coomassie Blue. For more information, see [Azure's website.](https://azurebiosystems.com/azure-200/) ## NanoString **NanoString nCounter SPRINT** ![NanoString nCounter SPRINT](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/nanostringncountersprint.jpg?itok=FZbB-g-W) For more information, [see NanoString's website](https://nanostring.com/products/ncounter-analysis-system/sprint-profiler/?gclid=Cj0KCQiAwJWdBhCYARIsAJc4idCG5ixsQcaKvqthnhgebi0xLgFQUy1s391ADxEcs2wsYg0dXHLnYq0aAuzzEALw_wcB).