#  Illumina Instruments 

 



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Before beginning a project, please [request a consultation ](/consultation-request-form)to discuss your experiment.

When Bauer Core performs sample quantification, we guarantee to deliver at least the lowest number in our advertised output range. While we are happy to accept runs with custom primers, we do not offer a yield guarrantee for those runs.

### **Custom Primers**

- **NextSeq 500/MiSeq:** We require 30 uL at 100 uM to either spike into the Illumina primers or to run with custom primers only. Indicate these details in your submission request.
- **NextSeq 1000:** If you want to **spike your primers** into the Illumina primers you will need to **purchase these directly from Illumina** and submit them with your sample for sequencing. Otherwise, we'll use your custom primer only for sequencing. Indicate these details in your submission request. 
    - Purchase kit: **20046115 - NextSeq 1000/2000 Read and Index Primers** [(more information at Illumina.com)](https://www.illumina.com/products/by-type/sequencing-kits/cluster-gen-sequencing-reagents/nextseq-1000-2000-reagents.html)
- **NovaSeq X Plus:** [Inquire](mailto:illumina_submission-list@lists.fas.harvard.edu)

Please note that custom primer runs are performed "at risk". While we have a great track record of successful custom primer runs, we cannot guarantee yield.



 

##  Illumina Sequencers 

 



 Illumina NovaSeq X Plus Illumina NextSeq 1000 Illumina NextSeq 500 Illumina MiSeq 

## Illumina NovaSeq X Plus

 

 

Sort   ![Illumina NovaSeq X Plus](/sites/g/files/omnuum7141/files/styles/hwp_1_1__100x100_scale/public/bsctestsite/files/illumina_novaseq_x_plus.jpg?itok=bHAKRwYW) 

 



- Single Read or Paired End reads
- Option to run different samples on each lane
- **Three Flow Cell Types & Available Kits:**
    - 1.5B: 2 lanes, 675 - 750 Million clusters/lane
        - 100, 200, 300 cycle
    - 10B: 8 lanes, 1.12 - 1.25 Billion clusters/lane
        - 100, 200, 300 cycle
    - 25B: 8 lanes, 2.81 – 3.12 Billion clusters/lane
        - 300 cycle









 



 

 

 

## Illumina NextSeq 1000

 

 

Sort   ![Illumina NextSeq1000 Instrument](/sites/g/files/omnuum7141/files/styles/hwp_1_1__360x360_scale/public/bsctestsite/files/nextseq_1000.jpg?itok=eDM7b1oF) 

 



- Single Read or Paired End reads up to 300 bp long
- **Two Flow Cell Types:**
    - P1: 90 - 100 M clusters
    - P2: 360 - 400 M clusters
- **Kits available (cycles used for sequence and index reads):**
    - 100 cycle kit: up to 138 bases
    - 200 cycle kit: up to 238 bases (P2 only)
    - 300 cycle kit: up to 338 bases
    - 600 cycle kit: up to 638 bases









 



 

 

 

## Illumina NextSeq 500

 

 

Sort   ![Illumina NextSeq 500](/sites/g/files/omnuum7141/files/styles/hwp_1_1__360x360_scale/public/bsctestsite/files/illumina_nextseq500.jpg?itok=udINvWFp) 

 



- Single Read or Paired End reads up to 150 bp long
- **Two Flow Cell Types:**
    - High: 300 - 400 M clusters
    - Mid: 100 - 130 M clusters
- **Kits available (used for sequence and index reads):**
    - 75 cycle kit: up to 92 bases (High Output only)
    - 150 cycle kit: up to 166 bases
    - 300 cycle kit: up to 316 bases









 



 

 

 

## Illumina MiSeq

 

 

Sort   ![Illumina MiSeq](/sites/g/files/omnuum7141/files/styles/hwp_1_1__360x360_scale/public/bsctestsite/files/illumina_miseq.jpg?itok=9lIP7mfz) 

 



- Single Read or Paired End reads up to 300 bp long
- **Four flow cell types:**
    - Nano: up to 1 M clusters
    - Micro: 4 - 5 M clusters
    - v2: 12 - 15 M clusters
    - v3: 20 - 25 M clusters