#  Submitting Samples for PacBio 

 



 download 

 [### Download LRS RNA Form 

 ](/resource/formlrsrnaxlsx) 

 

 download 

 [### Download LRS DNA Form 

 ](/resource/formlrsdnaxlsx) 

 

 download 

 [### Download LRS Sequencing Only Form

 ](/resource/formlrssequencingonlyxlsx) 

 

 

 

 

 

 

 

 

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1\. Consult with Core Staff to pick the best Run Type for your project

2\. Complete an online sample submission [here](https://bauer-minilims.rc.fas.harvard.edu/minilims/plugins/Core/make_form.php?type=Reagent_Request&name=new).

3\. Place samples in a provided zip-top bag with a completed submission slip.

4\. Log your sample drop-off on the paper log.

5\. Place the bag into the “sequencing drop-off" submission freezer in the hall.

**Turnaround Times:**

- Library Prep & Sequencing: Library prep is 4 weeks after samples pass our pre-prep QC which can take up to 2 weeks. Sequencing is an additional 2 weeks. [Email us](mailto:illumina_submission-list@lists.fas.harvard.edu) if you'd like a better estimate.
- Sequencing Only: 2 weeks.

SortPrep TypeInput

Material

Input

Concentrations

(ng/ul)

Volume

Requirements

(ul)

SRE XS

>1.5 ug gDNA

25 - 150 

60

SRE

>1.5 ug gDNA

50 - 150

60

Megaruptor

>1 ug gDNA

1 - 150

65 - 500

Whole Genome

SMRTbell v3.0



>1 ug of DNA w/o SRE

>1.5 ug w/SRE



3 - 39

100 - 130

IsoSeq SMRTbell v3.0

Starting Material: RNA



450 ng, RIN >7

45 

10

IsoSeq SMRTbell v3.0

Starting Material: cDNA



160-500 ng

4 - 12.5

40







 

##  Northwest Building Hours 

 Building doors are Open 7AM- 7PM Monday-Friday. For after-hours access, email the [Bauer Core Team](mailto:illumina_submission-list@lists.fas.harvard.edu).



 

##  Drop-off & Pick-up 

Sample drop-off/pick-up NWL B2 level outside the core.   ![Bauer Core Drop-off Station](/sites/g/files/omnuum7141/files/styles/hwp_1_1__360x360_scale/public/bsctestsite/files/img_6511_01.jpg?itok=fiQmZYur)