#  QC and Other Services 

 



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The Bauer Core offers a variety of services to assess the quality of your samples and prepare samples for downstream services or methods. Determining the initial quality of your samples is an important step to ensure that sequencing results are accurate and produce high-quality reads. Please see the list below to determine which QC best fits your needs.

[Reference the guide here to submit in LockBox.](https://drive.google.com/drive/folders/1kTXqgZZwqFCUWbIq78uNWzXLgH_1SlBr?usp=sharing)



 

##  Follow these steps to submit all new requests 

1. [Have a consultation](/consultation-request-form "Consultation & Estimate Request Form") for all new projects
2. [Sign up ](/harvard-bauer-core-lockbox-lims "Harvard Bauer Core: LockBox LIMS")for a LockBox Account
3. Prepare yoursample details 
    1. NOTE: You will download our template to upload into LockBox - no other formats accepted
4. Submit your request(s) - [use the references here as a guide ](https://drive.google.com/drive/folders/1kTXqgZZwqFCUWbIq78uNWzXLgH_1SlBr?usp=sharing)
5. Drop your samples off and log them - [drop-off details here ](https://bauercore.fas.harvard.edu/sample-drop)



 

##  How to Submit your Samples 

1. **<24 samples -** Can be should be in **1.5 mL or 2 mL Eppendorf** (or equivalent) **tubes**.
    1. ***Do not*** **submit samples in PCR tubes (0.2 mL) or 0.5 mL tubes**
2. **>24 samples**
    1. Submit samples in **clearly labeled PCR strip tubes or**
    2. **96-well plate** order should match the sample list in the index list
        1. ###### Array samples by column in a **semi or full-skirted 96-well plate with a foil seal** 
            
            
            1. *No empty wells between samples. In full column A->H format*
            2. Note: the core will provide this upon request



 

##  Turnaround Times 

Typically 2 weeks from the day all information is received and details of service(s) are confirmed.



 

##  QC Services 

If you are unsure which methods or instruments would be best for your sample, please reach out to [Core Staff](mailto:illumina_submission-list@lists.fas.harvard.edu) for a consultation.

 

 



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###    Pooling Libraries Before Pre-Sequencing QC  expand\_more  

 

**Pooling: Pool libraries equimolar from TapeStation data**- Tapestation data is used to determine the nM concentration of final libraries before pooling 
    - Submit a *TapeStation service request* quantity will be the number of submitted libraries for pooling
- Unless stated otherwise, libraries are pooled equimolar
- Once pooled, the final pool will enter our Pre-Sequencing QC queue. [See here for details.](/pre-sequencing-qc)



 

 

 



###    Size and Concentration Analysis  expand\_more  

 

Determining the size and concentration of your sample can help you determine which library preparation or sequencing flow cell may be best suited for your sample type. TapeStation is typically the best option for most samples, however very low concentration samples may require the use of a Bioanalyzer. Our new Femto Pulse will aid in very accurate size determination for both extremely low concentration RNA and high molecular weight DNA. [See here for more instrument details.](/instruments-at-the-bauercore)

 

 

 



###    Shearing/Size Selection  expand\_more  

 

Shearing samples is particularly important for long-read sequencing. Although ONT and Pac-Bio are able to handle longer molecules, making sure your samples are the within a specific size range will optimize the technology. Magnetic Bead cleanups are another option to select a specific region of your DNA product. Technologies available at the Core include Covaris, G-Tubes, Megaruptor, and Pippen Prep systems. [See here for more instrument details.](/instruments-at-the-bauercore)

 

 

 



###    Quantification & Normalization  expand\_more  

 

 Getting an accurate quantification of your samples is key to optimizing protocols to fit your samples. Normalization of your sample concentrations then ensures that each sample receives the same ratio of reagent mix in downstream protocols. For low quantities of samples, the Core offers qubit and nanodrop, while for larger sample sets, we support several plate readers including an Spectramax i3. [See here for more instrument details.](/instruments-at-the-bauercore)