Sample Analysis and QC

To learn about any of these instruments, please contact Claire Hartmann (claire@cgr.harvard.edu).

Agilent TapeStation 2200

The TapeStation rapidly analyzes the size and quantity of RNA and DNA samples. Tapes allow for small-scale gel electrophoresis using very little sample. Each tape can analyze up to 16 samples which may be run all together or at different times over the course of two weeks. For more information, see http://www.chem.agilent.com.

D1000 TapeStation Protocol NEW

HS D1000 TapeStation Protocol NEW

HS D5000 TapeStation Protocol NEW

gDNA TapeStation Protocol NEW

RNA TapeStation Protocol NEW

HS RNA TapeStation Protocol NEW

BioAnalyzer

Agilent Bioanalyzer 2100

The BioAnalyzers can be used to rapidly analyze RNA, DNA, and proteins. These instruments use microfluidic technology to perform automated quantification, and sizing of samples.

For more information, see http://www.chem.agilent.com.

Bioanalyzer RNA Nano Protocol UPDATED 01/2017

Bioanalyzer RNA Pico Protocol UPDATED 01/2017

Bioanalyzer HS DNA Protocol UPDATED 01/2017

Nanodrop

NanoDrop ND-1000 Spectrophotometer

The Nanodrop is a UV-Visible Spectrophotometer designed to read the absorbance of 1-2 ┬Ál samples without a cuvette. Most samples can be run without dilution. It has a wavelength range of 220-750nm. For more information see http://www.nanodrop.com.

NanoDrop Protocol

Quibit

Qubit Fluorimeter

The Qubit enables precise quantification of dsDNA, ssDNA, RNA and protein using a fluorescent dye. For more information see http://www.invitrogen.com/.

Qubit Protocol