#  Sequencing 

 



 [### Next Generation Sequencing (NGS)

 ](/next-generation-sequencing) 

 

 [### Long Read Sequencing (LRS)

 ](/long-read-sequencing) 

 

 [### Request a Consultation or an Estimate

 ](/consultation-request-form) 

 

 

 

 

 

 

 

 

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We support traditional Next-Generation Illumina, Long-Read Pac-Bio, and Oxford Nanopore Technologies (ONT) sequencing projects. Click on the links below for a brief video overview of each technology.

[Request a consultation before submitting your project.](/consultation-request-form "Consultation & Estimate Request Form") This is especially important for new users of the core.

Please [opt into our sequencing email list](https://web.lists.fas.harvard.edu/mailman/lists/high_throughput_sequencing@lists.fas.harvard.edu/) to receive important updates and information that may impact your project.



 

 Illumina PacBio Oxford Nanopore Technologies 

## Illumina

 

 

 [Illumina](https://www.youtube.com/watch?v=fCd6B5HRaZ8) instruments offer ultra-high-throughput sequencing known for its scalability and speed. Multiple samples can be indexed (barcoded) and combined to make the most of the system's output.

 **Applications include:**- Whole-genome sequencing
- Deep target region sequencing
- Gene expression analysis



 



 

 

 

## PacBio

 

 

 [Pac-Bio](https://www.youtube.com/watch?v=_lD8JyAbwEo) offers HiFi reads with an accuracy rate of over 99.9%, providing the quality of Illumina sequencing with the long reads needed to answer your research questions.

 **Applications include:**

- Genome assembly
- Genome mapping
- Iso-Seq



 



 

 

 

## Oxford Nanopore Technologies

 

 

 [ONT](https://www.youtube.com/watch?v=RcP85JHLmnI) instruments can directly sequence DNA or RNA and can deliver over 100 Gb of data per run. Multiple samples can be barcoded and combined to maximize a flow cell. Two flowcell options also allow researchers to prioritize the quantity or quality of data depending on research needs.

 **Applications include:**

- Differentiation of isoforms
- Structural variant resolution
- Genome assembly