#  Starting Material: RNA 

 



##  Watchmaker RNA Libray Preps 

- **Total RNA** - 0.2-20 ng/μL 
    - **20 μL** of RNase-free water
- 96-well full-skirted plate in column format with foil seal
    - Core will provide upon request
    - Label plate with name and date

[See here if you have legacy data using one of our previous assay offerings. ](https://bauercore.fas.harvard.edu/legacy-projects)

 

 



 ### Stranded, low to high input

- [Watchmaker mRNA Library Prep Kit](https://watchmakergenomics.com/portfolio/rna-solutions/mrna/) - 2.5 ng - 200 ng
- [Watchmaker RNA Library Prep Kit](https://watchmakergenomics.com/portfolio/rna-solutions/rna-prep/) - 0.25 ng -20 ng
- [Watchmaker RNA Library Prep Kits with Polaris™ Depletion](https://watchmakergenomics.com/portfolio/rna-solutions/rna-with-polaris/) - 1 ng - 200 ng



 

 ### Additional Ribosomal Depletion Options

- [QIAseq FastSelect -rRNA HMR Kits](https://www.qiagen.com/us/products/discovery-and-translational-research/next-generation-sequencing/rna-sequencing/ribosomal-rna-and-globin-mrna-removal/qiaseq-fastselect-rrna-hmr-kits?catno=334386)
- [QIAseq FastSelect Epidemiology Kits](https://www.qiagen.com/us/products/discovery-and-translational-research/next-generation-sequencing/rna-sequencing/ribosomal-rna-and-globin-mrna-removal/qiaseq-fastselect-epidemiology-kits)



 

  

 

 

 

 

##  Takara Smart-Seq mRNA LP with UMIs Libray Prep 

- Stranded, sorted single cells or bulk RNA
- 96-well full-skirted plate in column format with foil seal
    - Core will provide upon request
    - Label plate with name and date

[See here if you have legacy data using one of our previous assay offerings. ](https://bauercore.fas.harvard.edu/legacy-projects)

 

 



 ### 1/4 Volume Requirements 

- **Total RNA** - 10 pg - 25 ng
    - **2.5 μL** of RNase-free water
- Intact Cells - 1-250
    - 3.1 μL of CDS Sorting Solution (provided by core)



 

 ### Full Volume Requirements

- **Total RNA** - 10 pg - 100 ng
    - **20 μL** of RNase-free water
- Intact Cells - 1-1000
    - 12.5 μL of CDS Sorting Solution (provided by core)



 

  

 

 

 

 

 

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 download 

 [### Download NGS RNA Form 

 ](/resource/formngsrnaxlsx)