#  Submitting Samples -Bulk Library Prep & Sequencing 

 



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[Reference the guide here to submit in LockBox.](https://drive.google.com/drive/folders/1kTXqgZZwqFCUWbIq78uNWzXLgH_1SlBr?usp=sharing)



 

##  Follow these steps to submit all new requests 

1. [Have a consultation](/consultation-request-form "Consultation & Estimate Request Form") for all new projects
2. [Sign up ](/harvard-bauer-core-lockbox-lims "Harvard Bauer Core: LockBox LIMS")for a LockBox Account
3. Prepare yoursample details 
    1. NOTE: You will download our template to upload into LockBox - no other formats accepted
4. Submit your request(s) - [use the references here as a guide ](https://drive.google.com/drive/folders/1kTXqgZZwqFCUWbIq78uNWzXLgH_1SlBr?usp=sharing)
5. Drop your samples off and log them - [drop-off details here ](https://bauercore.fas.harvard.edu/sample-drop)



 

##  How to Submit your Samples or Pool(s) 

**Individual samples**

1. **<24 samples -** Can be should be in **1.5 mL or 2 mL Eppendorf** (or equivalent) **tubes**.
    1. ***Do not*** **submit samples in PCR tubes (0.2 mL) or 0.5 mL tubes**
2. **>24 samples**
    1. Submit samples in **clearly labeled PCR strip tubes or**
    2. **96-well plate** order should match the sample list in the index list
        1. ###### Array samples by column in a **semi or full-skirted 96-well plate with a foil seal** 
            
            
            1. *No empty wells between samples. In full column A->H format*
            2. Note: the core will provide this upon request



 

##  Turnaround Times 

Library preps are 3 weeks from the day QC and inputs are confirmed.



 

##  Volumes and Concentrations 

- Minimum of 20 uL per sample 
    - If you have more it is best to submit it and request pick up if you want the remaining volume
    - If you are submitting <20 uL note this in the "notes" section of your request
- RNA - Nuclease-free water
- DNA- Nuclease-free water or buffer with no EDTA
- Concentrations should be noted in the request, even an estimate in the notes 
    - Such as <1 ng/uL, <10 ng/uL, >100 ng/uL
    - There is no max, the minimum for each can be found on their respective pages



 

 

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 Attachments- [  list\_alt  Form\_NGS\_DNA.xlsx ](/sites/g/files/omnuum7141/files/2025-05/Form_NGS_DNA.xlsx)
- [  list\_alt  Form\_NGS\_RNA.xlsx ](/sites/g/files/omnuum7141/files/2025-05/Form_NGS_RNA.xlsx)
 
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