Instruments at the Bauer Core

To learn about any of these instruments, please contact the Core Staff.

Nucleic Acid Shearing

Covaris S220

The Covaris S220 system's controlled, reproducible DNA shearing method generates fragments with tight size distributions centered from 100 to 5000 bp. To request Covaris shearing service, please email this form to Core Staff.

For more information, see Covaris' website.

Fisher 550 Sonicator

Used for DNA fragmentation and cell disruption.

For more information, see Fisher Scientific's website.

Fisher 550 Sonicator Protocol

Diagenode Megaruptor 3

Megaruptor3 For more information, see Diagenode's website.

Tissue Dissociation and Homogenization

GentleMACS Octo Dissociator

The GentleMACS is a benchtop instrument for automated tissue dissociation for single-cell suspensions or tissue homogenization for RNA isolation of up to eight samples at a time. The instrument uses specially designed disposable tubes that depend on your application. Typical samples used on the instrument include human or mouse tumors, neural tissues, and heart or brain samples, and it can also handle plant samples for nuclei isolation. Researchers can be trained to use the instrument independently. To learn about using this instrument, please Core Staff.

For more information, see Miltenyi's website.

Imaging Systems

Azure Sapphire Imager

sapphire This multimode imager acquires fluorescent, chemiluminescent, and phosphor images with a resolution down to 10 microns. Fluorescent detection uses 4 lasers/filters: ex 488/em 518 bp22, ex 520/em 565 bp24, ex 658/em 710 bp40, ex 785/em 832 bp37. RGB fluorescent signal is captured using a PMT, while NIR is captured with an avalanche photodiode. Chemiluminescent and visible images are captured with a high-resolution CCD camera.

For more information, see Azure's website.

Azure c200 Imager

This gel imager uses UV for Ethidium bromide-stained DNA gels, blue light for SYBRSafe or similar dyes, and white light for Silver stain or Coomassie Blue.

For more information, see Azure's website.

NanoString

NanoString nCounter SPRINT

For more information, see NanoString's website.

Nucleic Acid Isolation

AutoGenPrep 965

The AutoGenPrep 965 performs completely automated DNA extraction from tissue, blood or plasmids/BACs in up to four 96-well plates at a time. To learn about using this instrument, please contact Core Staff.

For more information about Autogen, see here.

AutoGen Plasmid Protocol

AutoGen Tissue Protocol

Fragment Analysis and Quantification

Agilent TapeStation 4200

Electrophoresis, Sample Quality Control, TapeStation | Agilent

The TapeStation rapidly analyzes the size and quantity of RNA and DNA samples. Tapes allow for small-scale gel electrophoresis using very little sample. Each tape can analyze up to 16 samples which may be run all together or at different times over two weeks.

For more information, see Agilent's website.

Agilent TapeStation 4200

The Femto Pulse analyzes samples using pulsed-field capillary electrophoresis. It is ideal for analyzing High Molecular Weight gDNA and low-concentration RNA samples.

For more information, see Agilent's website.

Agilent Bioanalyzer 2100

The Bioanalyzer uses microfluidic technology to perform automated quantification and sizing to analyze RNA, DNA, and proteins..

For more information, see Agilent's website.

Qubit Fluorometer

The Qubit enables precise quantification of dsDNA, ssDNA, RNA, and protein using a fluorescent dye.

For more information, see Thermo Fisher's website.

Spectrophotometers- Sample Purity

NanoDrop ND-1000 Spectrophotometer

Thermo Scientific NanoDrop 1000 | GMI - Trusted Laboratory Solutions The Nanodrop is a UV-Visible Spectrophotometer designed to read the absorbance of 1-2 µl samples without a cuvette. Most samples can be run without dilution. It has a wavelength range of 220-750nm.

For more information, see Thermo Fisher's website.

Denovix DS-11

DS-11

The DS-11 is a UV-Visible Spectrophotometer capable of measuring the absorbance of samples using only 1 µl. It has a wavelength range of 190-840nm.

For more information, see Denovix's website.

Automated Size Selection

Blue Pippin & Pippin Prep

Sage Sciences Blue Pippin & Pippin Prep Instruments Sage Science's pippin instruments perform automated size selection for many sample types, such as High Molecular Weight gDNA and tight library size selection.