Sequencing

Next Generation Sequencing (NGS)

Long Read Sequencing (LRS)

Request a Consultation or an Estimate

We support traditional Next-Generation Illumina, Long-Read Pac-Bio, and Oxford Nanopore Technologies (ONT) sequencing projects. Click on the links below for a brief video overview of each technology. 

Request a consultation before submitting your project. This is especially important for new users of the core. 

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Illumina

Illumina instruments offer ultra-high-throughput sequencing known for its scalability and speed. Multiple samples can be indexed (barcoded) and combined to make the most of the system's output.

  Applications include:
  • Whole-genome sequencing
  • Deep target region sequencing
  • Gene expression analysis

PacBio

Pac-Bio offers HiFi reads with an accuracy rate of over 99.9%, providing the quality of Illumina sequencing with the long reads needed to answer your research questions.

 

Applications include:

  • Genome assembly
  • Genome mapping
  • Iso-Seq

Oxford Nanopore Technologies

ONT instruments can directly sequence DNA or RNA and can deliver over 100 Gb of data per run. Multiple samples can be barcoded and combined to maximize a flow cell. Two flowcell options also allow researchers to prioritize the quantity or quality of data depending on research needs.

 

Applications include:

  • Differentiation of isoforms
  • Structural variant resolution
  • Genome assembly