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Bulk Preps

The Core will take your starting DNA/RNA:
  1. Quantify starting material
  2. TapeStation on starting material (if applicable) 
  3. Normalize input (if applicable)
  4. Perform library preparation 
  5. Quantify final libraries 
  6. Check library quality on TapeStation
  7. Pool based on TapeStation or Picogreen results
  8. Kapa qPCR on the pool 
  9. Sequencing
  10. Demultiplexed Data Email
See the table below and the vendor protocols to determine which preparation may be best for your samples and schedule a more in-depth consultation with the Core Staff. 
 
Select your starting material for library prep sample submission requirements in the section navigation window on the left-hand side. 

RNA Library Preparation

Kit

 Low Concentration Compatible? 

 Low Volume Compatible? 

Chemistry

Best Used For

 Multiplexing available? 

*Kapa mRNA Hyper Prep 

No

No

Stranded, PolyA Selection

Intact, concentrated RNA

Yes

 *Kapa RNA Hyper Prep with Riboerase 

No

No

Stranded, Ribosomal Depletion

Intact, concentrated RNA

Yes

**Smart Seq v4

Yes & Single Cell

Yes

Unstranded, random priming with tagmentation 

Intact, low concentration RNA

Yes

Nanopore Direct RNA

No

No

Ligation

 Intact, low concentration mRNA

Yes

*15th of the month deadline
**1st of the month deadline
 

DNA Library Preparation

Kit

Low Concentration Compatible?

Low Volume Compatible?

Chemistry

Best Used For

Multiplexing available?

**Illumina DNA Yes No Tagmentation Intact, DNA Yes
**Nextera XT
Yes
Yes
Tagmentation
Intact, Low concentration input DNA
Yes
*Kapa DNA Hyper Plus
Yes
Yes
Ligation, Enzymatic Fragmentation
Intact, DNA
Yes
Nanopore Direct DNA LSK 109
No
No
Ligation
Barcoding
High output, good quality data
Yes
Nanopore Direct DNA LSK 110
No
No
Ligation
High output, good quality data
No
Nanopore Direct DNA LSK 112
No
No
Ligation
Low output, ultra-high quality data
No
*15th of the month deadline
**1st of the month deadline