Submitting Samples -Bulk Library Prep & Sequencing

1. Have a consultation for all new projects 
2. Sign up for a LockBox Account 
3. Prepare yoursample details 
   1. NOTE: You will download our template to upload into LockBox - no other formats accepted 
4. Submit your request(s) - use the references here as a guide 
5. Drop your samples off and log them - drop-off details here 

Individual samples

1. <24 samples - Can be should be in 1.5 mL or 2 mL Eppendorf (or equivalent) tubes.
   1. Do not submit samples in PCR tubes (0.2 mL) or 0.5 mL tubes
2.  >24 samples
   1. Submit samples in clearly labeled PCR strip tubes or 
   2. 96-well plate order should match the sample list in the index list

      1. Array samples by column in a semi or full-skirted 96-well plate with a foil seal 

         1. No empty wells between samples. In full column A->H format 
         2. Note: the core will provide this upon request 

Library preps are 3 weeks from the day QC and inputs are confirmed. 

• Minimum of 20 uL per sample 
  • If you have more it is best to submit it and request pick up if you want the remaining volume
  • If you are submitting <20 uL note this in the "notes" section of your request
• RNA - Nuclease-free water
• DNA- Nuclease-free water or buffer with no EDTA
• Concentrations should be noted in the request, even an estimate in the notes 
  • Such as <1 ng/uL, <10 ng/uL, >100 ng/uL
  • There is no max, the minimum for each can be found on their respective pages