NGS Library Preparation

NGS Library Preparation

High Throughput Library Preparation
   Price per 8 samples (1 full column of a 96 well plate), does not include Sequencing
Recommended Add-on Services Listed Below
High-Throughput Library Prep Service
Harvard Account Code
price per 8
PO Payment
price per 8
Kapa mRNA Hyperprep (incl. polyA selection) $687 $962
Illumina DNA (Nextera Flex) 1/4 volume $232 $325
Kapa DNA HyperPlus 1/4 volume
$206
$288
SmartSeq v4 + Nextera XT 1/4 volume $400 $560
Kapa RNA Hyperprep with RiboErase
$1,475
$2,065
Nextera XT 1/4 volume
$216
$302
 QC Add-ons For Full-Service Library Preparation
Most common services listed, this is not a comprehensive list 
See QC & Reagent Fees above for additional services
QC Service
 Harvard Account Code 
 PO Payment 
*Input Sample Pre-Library Prep QC
Agilent TapeStation (per sample)
$16
$22
Post-Library Prep QC
(per sample)
$16
$22
Pre-Seqeuncing QC - per pool
(TapeStation and QPCR)
$36
$50
*Optional RNA QC Service - RIN data may be provided by the researcher
 
 Single Cell Library Preparations
 Harvard Account Code 
 PO Payment 
10x Cell Prep Test
(per scheduled test, up to 4 samples)
$174
$243
10x Single Cell 3' v3.1 Library Prep
(per sample, does not include sequencing)
$2,392
$3,349
10x Single Cell ATAC v2 Library Prep
(per sample, does not include sequencing)
$2,565
$3,591
10x Single Cell Multiome v1
ATAC + Gene Expression Library Prep
(per sample, does not include sequencing)
$3,936 
$5,510
Other 10x Single Cell Assays
(Fixed RNA (Flex), 5', VDJ, Feature Barcoding)
inquire
inquire
SmartSeq v4 + Nextera XT 1/4 volume 
(sorted cells up to 125 cells, price per 8 samples)
$400 
$560
SmartSeq v4 - See Flow Cytometry services to sort your cells into a 96-well plate
See Flow Cytometry Instrument Training & Usage Fees select "Staff-Run Services" tab

Additional QC for Full-Service 10x Assays

QC Service

 Harvard Account Code 

 PO Payment 

10x Cell Prep Test
(per scheduled test, up to 4 samples)
$174
$243
Post-Library Prep QC
(per sample)
$16
$22
 Pre-Seqeuncing QC - per pool 
(TapeStation and QPCR)
$36
$50