1. Consult with Core Staff to pick the best Prep Type for your project
2. Complete an online sample submission here.
3. Place samples in a provided zip-top bag with a completed submission slip.
4. Log your sample drop-off on the paper log.
5. Place the bag into the “sequencing drop-off" submission freezer in the hall.
Prep Type
|
Input
Requirements
|
*Volume
Requirements
(ul)
|
---|---|---|
LSK 114 Kit
Q20
|
1 ug HMW gDNA
|
48
|
NBD114.24
Barcoded Q20
|
400ng-1 ug
DNA per sample
|
12
|
SQK-PCS114
Q20 cDNA PCR
|
polyA mRNA: 10 ng
Total RNA: 500 ng
|
10
|
SQK-RNA004
direct RNA
|
polyA mRNA: 300 ng
Total RNA: 1ug
|
8
|
*Sample should be in TRIS buffer or water. |