1. Consult with Core Staff to pick the best Run Type for your project
2. Complete an online sample submission here.
3. Place samples in a provided zip-top bag with a completed submission slip.
4. Log your sample drop-off on the paper log.
5. Place the bag into the “sequencing drop-off" submission freezer in the hall.
|
Prep Type
|
Input
Material
|
Input
Concentrations
(ng/ul)
|
Volume
Requirements
(ul)
|
|---|---|---|---|
|
SRE XS
|
>1.5 ug gDNA
|
25 - 150
|
60
|
|
SRE
|
>1.5 ug gDNA
|
50 - 150
|
60
|
|
Megaruptor
|
>1 ug gDNA
|
1 - 150
|
65 - 500
|
|
Whole Genome
SMRTbell v3.0
|
>1 ug of DNA w/o SRE
>1.5 ug w/SRE
|
3 - 39
|
100 - 130
|
|
IsoSeq SMRTbell v3.0
Starting Material: RNA
|
450 ng, RIN >7
|
45
|
10
|
|
IsoSeq SMRTbell v3.0
Starting Material: cDNA
|
160-500 ng
|
4 - 12.5
|
40
|
