Submitting Samples for PacBio

Reference the guide here to submit in LockBox.

Follow these steps to submit all new requests

  1. Have a consultation for all new projects 
  2. Sign up for a LockBox Account 
  3. Prepare your sample information
    1. NOTE: You will download our template to upload into LockBox - no other formats accepted 
  4. Submit your request(s) - use the references here as a guide 
  5. Drop your samples off and log them - drop-off details here 

How to Submit your Samples or Pool(s)

  • Final sequencing pools or individual samples
  • <24 samples - should be in 1.5 mL or 2 mL Eppendorf (or equivalent) tubes.
  •  >24 samples
    • Submit samples in clearly labeled PCR strip tubes or 
    • 96-well plate order should match the sample list in the index list
  • Do not submit samples in PCR tubes (0.2 mL) or 0.5 mL tubes 

Turnaround Times

  • Library Prep & Sequencing: Library prep is 3 weeks after samples pass our pre-prep QC which can take up to 2 weeks. Sequencing is an additional 2 weeks. Email us if you'd like a better estimate.
  • Sequencing Only: 2 weeks.

Volumes and Concentrations

Prep Type

Input 

Material

Input

Concentrations

(ng/ul)

Volume

Requirements

(ul)

SRE XS
>1.5 ug gDNA
25 - 150 
60
SRE
>1.5 ug gDNA
50 - 150
60
Megaruptor
>1 ug gDNA
1 - 150
65 - 500

Whole Genome 

SMRTbell v3.0 

>1 ug of DNA w/o SRE

>1.5 ug w/SRE

3 - 39
100 - 130

IsoSeq SMRTbell v3.0

Starting Material: RNA

450 ng, RIN >7
45 
10

IsoSeq SMRTbell v3.0

Starting Material: cDNA

160-500 ng
4 - 12.5
40