Submitting Samples for PacBio
1. Consult with Core Staff to pick the best Run Type for your project
2. Complete an online sample submission here.
3. Place samples in a provided zip-top bag with a completed submission slip.
4. Log your sample drop-off on the paper log.
5. Place the bag into the “sequencing drop-off" submission freezer in the hall.
Turnaround Times:
- Library Prep & Sequencing: Library prep is 4 weeks after samples pass our pre-prep QC which can take up to 2 weeks. Sequencing is an additional 2 weeks. Email us if you'd like a better estimate.
- Sequencing Only: 2 weeks.
| Prep Type | Input Material | Input Concentrations (ng/ul) | Volume Requirements (ul) |
|---|---|---|---|
SRE XS | >1.5 ug gDNA | 25 - 150 | 60 |
SRE | >1.5 ug gDNA | 50 - 150 | 60 |
Megaruptor | >1 ug gDNA | 1 - 150 | 65 - 500 |
Whole Genome SMRTbell v3.0 | >1 ug of DNA w/o SRE >1.5 ug w/SRE | 3 - 39 | 100 - 130 |
IsoSeq SMRTbell v3.0 Starting Material: RNA | 450 ng, RIN >7 | 45 | 10 |
IsoSeq SMRTbell v3.0 Starting Material: cDNA | 160-500 ng | 4 - 12.5 | 40 |
Northwest Building Hours
Building doors are Open 7AM- 7PM Monday-Friday. For after-hours access, email the Bauer Core Team.
Drop-off & Pick-up