Submitting Samples for PacBio

1. Consult with Core Staff to pick the best Run Type for your project

2. Complete an online sample submission here.

3. Place samples in a provided zip-top bag with a completed submission slip.

4. Log your sample drop-off on the paper log.

5. Place the bag into the “sequencing drop-off" submission freezer in the hall.

 

Turnaround Times: 

  • Library Prep & Sequencing: Library prep is 4 weeks after samples pass our pre-prep QC which can take up to 2 weeks. Sequencing is an additional 2 weeks. Email us if you'd like a better estimate.
  • Sequencing Only: 2 weeks.
Prep Type

Input 

Material

Input

Concentrations

(ng/ul)

Volume

Requirements

(ul)

SRE XS
>1.5 ug gDNA
25 - 150 
60
SRE
>1.5 ug gDNA
50 - 150
60
Megaruptor
>1 ug gDNA
1 - 150
65 - 500

Whole Genome 

SMRTbell v3.0 

>1 ug of DNA w/o SRE

>1.5 ug w/SRE

3 - 39
100 - 130

IsoSeq SMRTbell v3.0

Starting Material: RNA

450 ng, RIN >7
45 
10

IsoSeq SMRTbell v3.0

Starting Material: cDNA

160-500 ng
4 - 12.5
40

Northwest Building Hours

Building doors are Open 7AM- 7PM Monday-Friday. For after-hours access, email the Bauer Core Team

Drop-off & Pick-up

Sample drop-off/pick-up NWL B2 level outside the core.
Bauer Core Drop-off Station