- Consult with or request an estimate from Core Staff to pick the best Run Type for your project.
- Determine your Pre-Sequencing QC option.
- Prepare your index list using our template.
- Complete an online sequencing submission.
-
Samples should be in 1.5 mL or 2 mL Eppendorf (or equivalent) Tubes.
- Do not submit samples in PCR tubes (0.2 mL) or 0.5 mL tubes
- If you need pooling, submit samples in clearly labled PCR strip tubes or 96 well plate order should match sample list in the index template above.
- Place samples in a provided zip-top bag with the completed submission slip.
- Log your sample drop-off on the paper log.
- Place the bag into the “sequencing drop-off" submission freezer in the hall.
Note: See here if you would like library preparation service before sequencing.
|
Run Type
|
Recommended
[nM]
|
*Minimum
[nM]
|
**Minimum
Volume (ul)
|
|---|---|---|---|
|
MiSeq (any type)
|
>4
|
2
|
12
|
|
NextSeq 500 (any type)
|
>2
|
0.5
|
12
|
| NextSeq 1000 (anyt type) | >2 | 2 | 25 |
|
NovaSeq SP/S1 full flow cell
|
3
|
1.5
|
110
|
|
NovaSeqSP/S1 single lane
|
2
|
1.5
|
28
|
|
NovaSeq S2 full flow cell
|
3
|
1.5
|
160
|
|
NovaSeq S2 single lane
|
2
|
1.5
|
32
|
|
NovaSeq S4 full flow cell
|
3
|
1.5
|
320
|
|
NovaSeq S4 single lane
|
2
|
1.5
|
40
|
* lower library concentrations will be run at risk**Add additional volume between 3-5 ul for Pre-Sequencing QC |
|||
 | index_list.xlsx | 103 KB |
| 10x_index_list.xlsx | 128 KB |
