Submit Samples for Illumina Sequencing

Reference the guide here to submit in LockBox.

Follow these steps to submit all new requests

  1. Have a consultation for all new projects 
  2. Sign up for a LockBox Account 
  3. Prepare your index list 
    1. NOTE: You will download our template to upload into LockBox - no other formats accepted 
  4. Submit your request(s) - use the references here as a guide 
  5. Drop your samples off and log them - drop-off details here 

How to Submit your Samples or Pool(s)

Submit Samples in the Following Formats: 

  • >16 Samples must be arrayed in a 96-well plate in full columns  (A-H) without spaces 
    • Label the side of the plate with your name, submission ID, and date
  • 4-16 samples/pools 
    • PCR strip tubes with caps -labeled with # on sample import template
  • 1-4 samples/pools
    • 1.5 mL tubes or strip tubes - clearly labeled including the # on the sample import template

  • Do not submit samples in PCR tubes (0.2 mL) or 0.5 mL tubes 

    *NOTE: Samples received in other formats will not be accepted and have to be rearrayed by the researcher. The Core is not responsible for any delays.

  • Do not submit samples in PCR tubes (0.2 mL) or 0.5 mL tubes 

Have custom primers? More Information Here!

Turnaround Times

  • Full Flow Cells: 

    • Most run within 2 weeks after qPCR
    • They will run within 2 weeks without qPCR 
      • Email us if you'd like a better estimate.

  • Single Lanes: 

    • Take longer to fill, but we expect the most popular options to run within 2-3 weeks. 
    • If a quick turnaround is your main concern, we can suggest other options, email us.

Volumes and Concentrations

 

Submission Volume and Concentration
Instrument and Run Type*[nM] of Pool*Volume per Lane/Flow Cell  
MiSeq or NextSeq 500 (any type) 
4 nM
20
NextSeq 1000 (any type)
4 nM 
40

NovaSeq X Plus 1.5B/5B/10B 

(any length) Per Lane

(1.5B- 2 Lanes, 

5B/10B- 8 Lanes per Flow Cell)

4 nM
40

NovaSeq X Plus 25B 

(any length) Per Lane

(8 Lanes per Flow Cell)

4 nM
60

*lower library concentrations will be run at risk

*If you need to submit samples <2 nM you should contact the sequencing team for additional guidance.

  • Submit your sequencing pool at the concentration and volume listed for your instrument and run type.
  • Note you should multiply volume by the number of lanes or flow cells you plan to sequence.
  • The core is not responsible for any delays if samples do not meet these requirements.