Submit Samples for Illumina Sequencing
Follow these steps to submit all new requests
- Have a consultation for all new projects
- Sign up for a LockBox Account
- Prepare your index list
- NOTE: You will download our template to upload into LockBox - no other formats accepted
- Submit your request(s) - use the references here as a guide
- Drop your samples off and log them - drop-off details here
How to Submit your Samples or Pool(s)
- Final sequencing pools or individual samples
- <24 samples - should be in 1.5 mL or 2 mL Eppendorf (or equivalent) tubes.
- >24 samples
- Submit samples in clearly labeled PCR strip tubes or
- 96-well plate order should match the sample list in the index list
Do not submit samples in PCR tubes (0.2 mL) or 0.5 mL tubes
Turnaround Times
Full Flow Cells:
- Most run within 2 weeks after qPCR
- They will run within 2 weeks without qPCR
- Email us if you'd like a better estimate.
Single Lanes:
- Take longer to fill, but we expect the most popular options to run within 2-3 weeks.
- If a quick turnaround is your main concern, we can suggest other options, email us.
Volumes and Concentrations
| Submission Volume and Concentration | ||
|---|---|---|
| Instrument and Run Type | *[nM] of Pool | *Volume per Lane/Flow Cell |
MiSeq or NextSeq 500 (any type) | 4 nM | 20 |
NextSeq 1000 (any type) | 4 nM | 40 |
NovaSeq X Plus 1.5B/5B/10B (any length) Per Lane (1.5B- 2 Lanes, 5B/10B- 8 Lanes per Flow Cell) | 4 nM | 40 |
NovaSeq X Plus 25B (any length) Per Lane (8 Lanes per Flow Cell) | 4 nM | 60 |
*lower library concentrations will be run at risk *If you need to submit samples <2 nM you should contact the sequencing team for additional guidance. | ||
- Submit your sequencing pool at the concentration and volume listed for your instrument and run type.
- Note you should multiply volume by the number of lanes or flow cells you plan to sequence.
- The core is not responsible for any delays if samples do not meet these requirements.