Submit Samples for Illumina Sequencing
- Consult with us or Request an estimate
- Download and complete our form
- Submit your form in minilims
- Upload it under supporting documents
- Note: If you have multiple files to submit, put all docs in one folder and zip it up to upload it
- Prepare your index list using our template
- Final sequencing pools should be in 1.5 mL or 2 mL Eppendorf (or equivalent) Tubes.
- Do not submit samples in PCR tubes (0.2 mL) or 0.5 mL tubes
- If you need pooling, submit samples in clearly labled PCR strip tubes or 96 well plate order should match sample list in the index templates.
- Submit your sample in the submission freezer in the hall
Note: See here if you would like library preparation service before sequencing.
Have custom primers? More Information Here!
Turnaround Times:
- Flow Cells: Most run within 2 weeks plus one additional week for Pre-Sequencing QC. Email us if you'd like a better estimate.
- Single Lanes: These can take longer to fill, but we expect the most popular options to run within the same timeframe. If quick turnaround is your main concern we can always offer other options for your run, email us for options.
| Submission Volume and Concentration | ||
|---|---|---|
| Instrument and Run Type | *[nM] of Pool | *Volume per Lane/Flow Cell |
MiSeq or NextSeq 500 (any type) | 4 nM | 20 |
NextSeq 1000 (any type) | 4 nM | 40 |
NovaSeq X Plus 1.5B/10B (any length) Per Lane (1.5B- 2 Lanes, 10B- 8 Lanes per Flow Cell) | 4 nM | 40 |
NovaSeq X Plus 25B (any length) Per Lane (8 Lanes per Flow Cell) | 4 nM | 60 |
*lower library concentrations will be run at risk *If you need to submit samples <2 nM you should contact the sequencing team for additional guidance. | ||
- Submit your sequencing pool at the concentration and volume listed for your instrument and run type.
- Note you should multiply volume by the number of lanes or flow cells you plan to sequence.
- The core is not responsible for any delays if samples do not meet these requirements.
Northwest Building Hours
Building doors are Open 7AM- 7PM Monday-Friday. For after-hours access, email the Bauer Core Team.
Drop-off & Pick-up