LRS: Long-Read Library Preparation & Sequencing
PacBio Sequel IIe Library Prep & Sequencing
| PacBio Sequel IIe Sequencing | ||
|---|---|---|
|
SMRT Cell ~ 20Gb
~20x coverage for 1 Gb Genome
|
Harvard
Account Code
|
PO
Payment
|
|
SMRT Cell 8M sequencing, per cell
|
$1,838
|
$2,573
|
|
PacBio Library Preparation
(per sample, does not include sequencing)
|
||
|---|---|---|
|
SMRTbell 3.0 Library Prep
|
Harvard Account Code
|
PO Payment
|
|
Whole Genome
(optional barcodes included)
|
$635
|
$898
|
|
IsoSeq
(optional barcodes included)
|
$337
|
$471
|
| 10x MAS-IsoSeq | $1,335 | $1,869 |
|
|
||
|
Recommended QC Services
|
||
|
QC Service, per sample
|
Harvard Account Code
|
PO Payment
|
|
Short Read Eliminator (SRE)
(per sample)
|
$93
|
$130
|
|
Megaruptor Shearing
(per sample)
|
$105
|
$146
|
|
Qubit and Nanodrop
(per sample)
|
$9
|
$13
|
Nanopore Library Prep & Sequencing
|
Oxford Nanopore Technologies (ONT) Sequencing
|
||||||
|---|---|---|---|---|---|---|
| PromethION ~ 80-90 Gb | MinION ~10 Gb | |||||
|
Per Flow Cell
~80-90x coverage
for 1 Gb Genome
|
Harvard
Account Code
|
PO
Payment
|
Per Flow Cell
~1x coverage
for 1 Gb Genome
|
Harvard
Account Code
|
PO
Payment
|
|
|
PromethION Sequencing
|
$1,300
|
$1,820
|
MinION Sequencing | $950 | $1,330 | |
|
Oxford Nanopore Library Prep
(per sample, does not include sequencing)
|
||
|---|---|---|
|
ONT Library Prep Kit
|
Harvard Account Code
|
PO Payment
|
|
Ligation Library
(LSK114)
|
$478
|
$669
|
|
Ligation Library with Native Barcodes
(SQK-NBD114.24)
|
$205
|
$287
|
|
10x Library
(SQK-PCS111)
|
$718
|
$1,006
|
|
Direct RNA Library Preparation
(RNA002)
|
$685
|
$958
|
|
PCR cDNA Library
(SQK-PCS111)
|
$809
|
$1,132
|
|
Barcoded PCR cDNA Library
(SQK-PCB111.24)
|
$273 | $382 |
|
Rapid Library
(SQK-RAD004)
|
$190
|
$266
|
|
Recommended QC Services
|
||
|
QC Service, per sample
|
Harvard Account Code
|
PO Payment
|
|
Qubit and Nanodrop
(per sample)
|
$9
|
$13
|
|
Covaris g-tube Shearing Service
(per sample)
|
$67
|
$94
|
|
Nuclease Flush + ReLoad
|
$150
|
$210
|
PacBio Revio Library Prep & Sequencing
|
PacBio Revio Sequencing
|
||
|---|---|---|
|
SMRT Cell ~ 90Gb
|
Harvard
Account Code
|
PO
Payment
|
|
SMRT Cell 25M sequencing, per cell
|
$2,425
|
$3,395
|
|
PacBio Library Preparation
(per sample, does not include sequencing)
|
||
|---|---|---|
|
SMRTbell 3.0 Library Prep
|
Harvard Account Code
|
PO Payment
|
|
Whole Genome
(optional barcodes included)
|
$635
|
$889
|
|
IsoSeq
(optional barcodes included)
|
$337
|
$471
|
|
10x MAS-IsoSeq
|
$1,335
|
$1,869
|
|
|
||
|
Recommended QC Services
|
||
|
QC Service, per sample
|
Harvard Account Code
|
PO Payment
|
|
Short Read Eliminator (SRE)
(per sample)
|
$93
|
$130
|
|
Megaruptor Shearing
(per sample)
|
$105
|
$146
|
|
Qubit and Nanodrop
(per sample)
|
$9
|
$13
|