HOME / LIBRARY PREP / SINGLE CELL PREPS /

10x Genomics

The 10x Chromium Next GEM technology partitions cells or nuclei into droplets containing uniquely barcoded beads called GEMs (Gel Bead-In Emulsions). The incorporation of a unique barcode into each cDNA/DNA fragment, allows each fragment to be traced back to the cell or nucleus of origin. By profiling thousands of cells simultaneously, the 10x Next GEM technology enables the unraveling of transcriptional and cellular heterogeneity within a cell population. The high number of profiled cells allows for the detection of even rare cell types within a complex sample.

Interested in feature barcoding? See our table below. 

10x Genomics end to end workflow image

10x at the Core

Library Preparation
We routinely run samples as a service on the Chromium System for single cell RNAseq, ATAC, and Multiome assays. We also provide service for additional 10x Genomics assays, if you do not see your assay of interest listed, contact us to inquire or  schedule an in-depth consultation to determine which preparation may be the best fit for your samples. 
Training
We also provide training for self-run use of the Chromium Controller instrument. Training consists of using non-functional reagents to learn to load the 10x chip. During run times, before and after training, we will review the protocol, cell prep, timing, and any other questions you may have. Each person must complete this training and submit a minilims request to gain access. Training can be anywhere between 1-2 hours, depending on the number of attendees. If you have >2 attendees, it is recommend to  split up into groups of 2 to keep the training length to 2 hours. 
 
For more information about the technology, please visit 10x Genomics.
To schedule a training, submit a request form. 

10x Genomics Feature Barcoding

These are “features” available for the barcoding of samples. There are a few available, some that are officially supported by 10x Genomics and others that are not. The Bauer Core can help to run both with the understanding that non-supported features have limited support. Below you will find the terminology, compatibly, and additional details. 
 
BioLegend Antibody Set
Antibody Based?
Feature
10x Genomics Assay
Supported by 10x?
Need to know Target?
Yes
Cell Surface Protein
&
Cell Hashing
None; Poly-A Compatible
No
Yes
Yes
Cell Surface Protein (CSP)
10x 3' v3.1 & Flex Single Plex 
Yes
Yes
Yes
 Cell Hashing (CH)
10x 3' v3.1 & Flex Single Plex 
No
Yes
Yes
Cell Surface Protein
&
Cell Hashing
10x 5' V(D)J v2 & Flex Multiplex 
Yes
Yes
No - Lipid Based Tag
Cell Multiplexing Oligo (CMO)
10x 3' v3.1 Only 
Yes
No