Starting Material: RNA

Watchmaker RNA Libray Preps

  • Total RNA - 0.2-20 ng/μL   
    • 20 μL of RNase-free water
  • 96-well full-skirted plate in column format with foil seal
    • Core will provide upon request
    • Label plate with name and date

 

See here if you have legacy data using one of our previous assay offerings. 

 

Takara Smart-Seq mRNA LP with UMIs Libray Prep

  • Stranded, sorted single cells or bulk RNA
  • 96-well full-skirted plate in column format with foil seal
    • Core will provide upon request
    • Label plate with name and date

See here if you have legacy data using one of our previous assay offerings. 

1/4 Volume Requirements

  • Total RNA - 10 pg - 25 ng
    • 2.5 μL of RNase-free water
  • Intact Cells - 1-250
    • 3.1 μL of CDS Sorting Solution (provided by core)

Full Volume Requirements

  • Total RNA - 10 pg - 100 ng
    • 20 μL of RNase-free water
  • Intact Cells - 1-1000
    • 12.5 μL of CDS Sorting Solution (provided by core)