Single-Cell Technologies

Individual cells can differ by size, protein levels, and expressed mRNA transcripts, even within nominally homogeneous cell populations. The Bauer Core has a variety of technologies to facilitate single-cell studies.

10x Chromium System

10x Genomics' Chromium technology partitions reactions into nanoliter-scale droplets containing uniquely barcoded beads called GEMs (Gel Bead-In EMulsions). This core technology can be used to partition single cells or high molecular DNA to prepare next generation sequencing libraries in parallel . We provide training and run samples as a service on the Chromium system. Please contact Claire Reardon to discuss your experimental plans. For more information about the technology, please visit 10xGenomics

Chromium Workflow

 

Fluidigm Systems

The Fluidigm Systems use microfluidic-based Integrated Fluidic Circuit (IFC) technology to enable rapid and reliable analysis of single cells. To learn more about our services, please contact Claire Reardon .

Fluidigm C1™  Single-Cell Auto Prep System

Fluidigm C1™ Single-Cell Auto Prep System employs microfluidic-based integrated fluidic circuits (IFCs) to isolate and prepare individual cells for genome and transcriptome analysis. Researchers can isolate cells, image them, and prepare template for sequencing or qPCR.

Bauer Core C1 Cell Prep Guide

Fluidigm Biomark™ HD System

Fluidigm Biomark HD System uses microfluidic-based integrated fluidic circuits (IFCs) to automate PCR reactions in nanoliter volumes, saving sample and reagent. The system has reliable production-scale throughput and single-cell sensitivity. It can accomodate a wide variety of sample types and chemistry choices and can run in either real-time or end-point read modes to enable many genomic applications.

Single-Cell Sorting

Flow Cytometry

Our cell sorters have the capability to sort single cells into multi-well plates. Please go the flow cytometry page to see a description of our cell sorters and contact information.