Single Cell Preps

10x Genomics

Sorted Cells

Individual cells can differ by size, protein levels, and expressed mRNA transcripts, even within nominally homogeneous cell populations. The Bauer Core supports both 10x Genomics and plate-based SMART-seq v4 technologies to facilitate single-cell studies. See the table below for a comparison of each RNA workflow.

For additional 10x assays, see our 10x Genomics assays page. 

Please request a consultation to discuss your experimental plans.

Method 10x Genomics Plate-based SMART-seq v4
Number of cells
thousands
hundreds
mRNA coverage
3’ or 5’ end
Full-length
Samples multiplexing
Yes
No
Single cell isolation
10x Chromium controller
FACS
Isolated cells storage
No
Yes
Limitations
3’ or 5’ end.  Isolated cells must be processed immediately
Low number of cells
Advantages
High number of cells. Multiplexing.
Full-length mRNA. Sorted cells can be stored at -80oC. Bulk cells (up to 250/well)
Applications
Heterogeneous cell population. Transcriptome. Epigenome. Multiome. Immune profiling (T or B cells). Expression of cell-surface proteins.
Transcriptome. Isoforms and rare, low abundance transcripts. Gene fusions.  Point mutations. Small pieces of dissected tissues/organisms.