Individual cells can differ by size, protein levels, and expressed mRNA transcripts, even within nominally homogeneous cell populations. The Bauer Core has a variety of technologies to facilitate single-cell studies.
10x Chromium System
10x Genomics' Chromium technology partitions reactions into nanoliter-scale droplets containing uniquely barcoded beads called GEMs (Gel Bead-In EMulsions). This core technology can be used to partition single cells or high molecular DNA to prepare next generation sequencing libraries in parallel . We provide training and run samples as a service on the Chromium system. Please contact Claire Reardon to discuss your experimental plans. For more information about the technology, please visit 10xGenomics.
Fluidigm C1™ Single-Cell Auto Prep System employs microfluidic-based integrated fluidic circuits (IFCs) to isolate and prepare individual cells for genome and transcriptome analysis. We offer limited support for the Fluidigm C1. To learn more, please contact Claire Reardon .
Fluidigm Biomark HD System uses microfluidic-based integrated fluidic circuits (IFCs) to automate PCR reactions in nanoliter volumes, saving sample and reagent. The system has single-cell sensitivity and can accomodate a wide variety of sample types and chemistry choices.