Illumina Instruments

Before beginning a project, please request a consultation to discuss your experiment.

When Bauer Core performs sample quantification, we guarantee to deliver at least the lowest number in our advertised output range. While we are happy to accept runs with custom primers, we do not offer a yield guarrantee for those runs.

Custom Primers

NextSeq 500/MiSeq: We require 30 uL at 100 uM to either spike into the Illumina primers or to run with custom primers only. Indicate these details in your submission request.
NextSeq 1000: If you want to spike your primers into the Illumina primers you will need to purchase these directly from Illumina and submit them with your sample for sequencing. Otherwise, we'll use your custom primer only for sequencing. Indicate these details in your submission request.
- Purchase kit: 20046115 - NextSeq 1000/2000 Read and Index Primers (more information at Illumina.com)
NovaSeq X Plus: Inquire

Please note that custom primer runs are performed "at risk". While we have a great track record of successful custom primer runs, we cannot guarantee yield.

Illumina Sequencers

Illumina NovaSeq X Plus

	Single Read or Paired End reads Option to run different samples on each lane Three Flow Cell Types & Available Kits: 1.5B: 2 lanes, 675 - 750 Million clusters/lane 100, 200, 300 cycle 10B: 8 lanes, 1.12 - 1.25 Billion clusters/lane 100, 200, 300 cycle 25B: 8 lanes, 2.81 – 3.12 Billion clusters/lane 300 cycle

Single Read or Paired End reads
Option to run different samples on each lane
Three Flow Cell Types & Available Kits:
- 1.5B: 2 lanes, 675 - 750 Million clusters/lane
  - 100, 200, 300 cycle
- 10B: 8 lanes, 1.12 - 1.25 Billion clusters/lane
  - 100, 200, 300 cycle
- 25B: 8 lanes, 2.81 – 3.12 Billion clusters/lane
  - 300 cycle

Illumina NextSeq 1000

	Single Read or Paired End reads up to 300 bp long Two Flow Cell Types: P1: 90 - 100 M clusters P2: 360 - 400 M clusters Kits available (cycles used for sequence and index reads): 100 cycle kit: up to 138 bases 200 cycle kit: up to 238 bases (P2 only) 300 cycle kit: up to 338 bases 600 cycle kit: up to 638 bases

Single Read or Paired End reads up to 300 bp long
Two Flow Cell Types:
- P1: 90 - 100 M clusters
- P2: 360 - 400 M clusters
Kits available (cycles used for sequence and index reads):
- 100 cycle kit: up to 138 bases
- 200 cycle kit: up to 238 bases (P2 only)
- 300 cycle kit: up to 338 bases
- 600 cycle kit: up to 638 bases

Illumina NextSeq 500

	Single Read or Paired End reads up to 150 bp long Two Flow Cell Types: High: 300 - 400 M clusters Mid: 100 - 130 M clusters Kits available (used for sequence and index reads): 75 cycle kit: up to 92 bases (High Output only) 150 cycle kit: up to 166 bases 300 cycle kit: up to 316 bases

Single Read or Paired End reads up to 150 bp long
Two Flow Cell Types:
- High: 300 - 400 M clusters
- Mid: 100 - 130 M clusters
Kits available (used for sequence and index reads):
- 75 cycle kit: up to 92 bases (High Output only)
- 150 cycle kit: up to 166 bases
- 300 cycle kit: up to 316 bases

Illumina MiSeq

	Single Read or Paired End reads up to 300 bp long Four flow cell types: Nano: up to 1 M clusters Micro: 4 - 5 M clusters v2: 12 - 15 M clusters v3: 20 - 25 M clusters

Single Read or Paired End reads up to 300 bp long
Four flow cell types:
- Nano: up to 1 M clusters
- Micro: 4 - 5 M clusters
- v2: 12 - 15 M clusters
- v3: 20 - 25 M clusters