FY24 Rates - Effective July 1,2023- June 30,2024
Before beginning a training session or submitting your samples for processing, you must provide your billing information. See our Billing Policies for details.
FY24 Rates
Flow Cytometry
Staff-Run Services
|
Staff-Run
|
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|---|---|---|
|
Service
|
Harvard Account
Code
|
PO
Payment
|
|
Sorting
|
$123/hr
|
$185/hr
|
|
Analyzer
|
$99/hr
|
$159/hr
|
Instrument Training
|
Instrument
|
Harvard Account
Code
|
PO
Payment
|
|---|---|---|
|
Analyzer
(LSRII or Fortessa)
(includes HTS autosampler)
(prerequisite: Intro to Flow Workshop)
|
$308
|
$462
|
|
FACS Aria or Biosorter
(prerequisite: Analyzer Training)
|
$862
|
$1,293
|
|
Multisizer
|
$201
|
$302
|
Training fees are per person
Self-Run Instrument Usage
|
Self-Run
|
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|---|---|---|
|
Service
|
Harvard Account
Code
|
PO
Payment
|
|
Sorting
(Aria,Biosorter, S3)
|
$46/hr
|
$68/hr
|
|
Aurora
|
$46/hr
|
$69/hr
|
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BD Analyzer or Multisizer
|
$31/hr
|
$47/hr
|
QC Services
Pooling
|
QC Service
|
Harvard Account Code
|
PO Payment
|
|---|---|---|
|
Pool libraries before qPCR
(TapeStation of each sample)
|
$16
|
$22
|
|
*Automated Pooling
(per plate, up to 4 pools)
|
$232
|
$325
|
|
*Quantification request is required for this service
|
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Quantification
|
QC Service
|
Harvard Account Code
|
PO Payment
|
|---|---|---|
|
Qubit and Nanodrop
(per sample)
|
$9
|
$13
|
|
Quantification
(up to 96 samples)
|
$94
|
$132
|
|
Quantify and Normalize
(up to 96 samples)
|
$434
|
$608
|
Size Selection
|
QC Service
|
Harvard Account Code
|
PO Payment
|
|---|---|---|
|
MagBead Cleanup
(per sample or pool)
|
$44
|
$62
|
|
MagBead Cleanup
(per plate)
|
$295
|
$413
|
|
Pippin Prep or Blue Pippin
Size Selection
(per sample)
|
$98
|
$137
|
|
Short Read Eliminator Kit
SRE w/ PacBio Prep
(per sample)
|
$93
|
$130
|
Shearing
|
QC Service
|
Harvard Account Code
|
PO Payment
|
|---|---|---|
|
Covaris Shearing
(per sample)
|
$50
|
$70
|
|
Megaruptor
(per sample)
|
$105
|
$146
|
|
G-Tube Shearing
(per sample)
|
$67
|
$94
|
Fragment Analysis & qPCR
|
QC Service
|
Harvard Account Code
|
PO Payment
|
|---|---|---|
|
Agilent TapeStation
(per sample or pool)
|
$16
|
$22
|
|
Pre-Sequencing QC
(TapeStation and QPCR)
(per sample or pool)
|
$36
|
$50
|
|
Agilent Bioanalyzer
(per sample or pool)
|
$38
|
$53
|
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FemtoPulse
(per sample)
|
$41
|
$58
|
DNA Extraction
|
Service
|
Harvard Account Code
|
PO Payment
|
|---|---|---|
|
High Molecular Weight
gDNA Extraction
(per sample)
|
$233
|
$326
|
Reagents for Purchase
|
Reagent
|
Harvard Account Code
|
PO Payment
|
|---|---|---|
|
D1000 Tape + use fee
|
$69/tape
|
$97/tape
|
|
D5000 Tape + use fee
|
$69/tape
|
$97/tape
|
|
High Sensitivity D1000 Tape + use fee
|
$92/tape
|
$128/tape
|
|
High Sensitivity D5000 Tape + use fee
|
$92/tape
|
$128/tape
|
|
gDNA Tape + use fee
|
$97/tape
|
$136/tape
|
|
RNA Tape + use fee
|
$68/tape
|
$95/tape
|
|
High Sensitivity RNA Tape + use fee
|
$71/tape
|
$101/tape
|
|
PCRClean DX Magnetic Beads (Aline), 1 mL
|
$6
|
$8
|
NGS Library Preparation
|
High Throughput Library Preparation
Price per 8 samples (1 full column of a 96 well plate), does not include Sequencing
Recommended Add-on Services Listed Below
|
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|---|---|---|
|
High-Throughput Library Prep Service
|
Harvard Account Code
price per 8
|
PO Payment
price per 8
|
| Kapa mRNA Hyperprep (incl. polyA selection) | $687 | $962 |
| Illumina DNA (Nextera Flex) 1/4 volume | $232 | $325 |
|
Kapa DNA HyperPlus 1/4 volume
|
$206
|
$288
|
| SmartSeq v4 + Nextera XT 1/4 volume | $400 | $560 |
|
Kapa RNA Hyperprep with RiboErase
|
$1,475
|
$2,065
|
|
Nextera XT 1/4 volume
|
$216
|
$302
|
|
QC Add-ons For Full-Service Library Preparation
Most common services listed, this is not a comprehensive list
See QC & Reagent Fees above for additional services
|
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|---|---|---|
|
QC Service
|
Harvard Account Code
|
PO Payment
|
|
*Input Sample Pre-Library Prep QC
Agilent TapeStation (per sample)
|
$16
|
$22
|
|
Post-Library Prep QC
(per sample)
|
$16
|
$22
|
|
Pre-Seqeuncing QC - per pool
(TapeStation and QPCR)
|
$36
|
$50
|
| *Optional RNA QC Service - RIN data may be provided by the researcher | ||
|
Single Cell Library Preparations
|
Harvard Account Code
|
PO Payment
|
|---|---|---|
|
10x Cell Prep Test
(per scheduled test, up to 4 samples)
|
$174
|
$243
|
|
10x Single Cell 3' v3.1 Library Prep
(per sample, does not include sequencing)
|
$2,392
|
$3,349
|
|
10x Single Cell ATAC v2 Library Prep
(per sample, does not include sequencing)
|
$2,565
|
$3,591
|
|
10x Single Cell Multiome v1
ATAC + Gene Expression Library Prep
(per sample, does not include sequencing)
|
$3,936
|
$5,510
|
|
Other 10x Single Cell Assays
(Fixed RNA (Flex), 5', VDJ, Feature Barcoding)
|
inquire
|
inquire
|
|
SmartSeq v4 + Nextera XT 1/4 volume
(sorted cells up to 125 cells, price per 8 samples)
|
$400
|
$560
|
|
SmartSeq v4 - See Flow Cytometry services to sort your cells into a 96-well plate
See Flow Cytometry Instrument Training & Usage Fees select "Staff-Run Services" tab
|
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|
Additional QC for Full-Service 10x Assays |
||
|---|---|---|
|
QC Service |
Harvard Account Code |
PO Payment |
|
10x Cell Prep Test
(per scheduled test, up to 4 samples)
|
$174
|
$243
|
|
Post-Library Prep QC
(per sample)
|
$16
|
$22
|
|
Pre-Seqeuncing QC - per pool
(TapeStation and QPCR)
|
$36
|
$50
|
MiSeq
|
MiSeq
|
||||||
|---|---|---|---|---|---|---|
| Nano v2 Flow Cell ~1 Million Reads |
Micro v2 Flow Cell ~4 Million Reads
|
|||||
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
|
|
MiSeq Nano
300 cycle
Flow Cell
|
$521
|
$729
|
|
MiSeq Micro
300 cycle
Flow Cell
|
$733
|
$1,026
|
|
MiSeq Nano
500 cycle
Flow Cell
|
$693
|
$970
|
|
|
|
|
|
MiSeq
|
||||||
|---|---|---|---|---|---|---|
|
MiSeq v2 Flow Cell ~15 Million Reads
|
MiSeq v3 Flow Cell ~25 Million Reads
|
|||||
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
|
|
MiSeq v2
50 cycle
Flow Cell
|
$1,156
|
$1,618
|
|
MiSeq v3
150 cycle
Flow Cell
|
$1,256
|
$1,759
|
|
MiSeq v2
300 cycle
Flow Cell
|
$1,412
|
$1,976
|
MiSeq v3
600 cycle
Flow Cell
|
$2,076
|
$2,907
|
|
|
MiSeq v2
500 cycle
Flow Cell
|
$1,619 | $2,266 | ||||
NextSeq 1000
|
NextSeq 1000
|
||||||
|---|---|---|---|---|---|---|
|
P1 Flow Cell ~90 Million Reads
|
P2 Flow Cell ~360 Million Reads
|
|||||
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
|
|
NextSeq P1
100 cycle
Flow Cell
|
$1,134
|
$1,587
|
|
NextSeq P2
100 cycle
Flow Cell
|
$1,638
|
$2,293
|
|
NextSeq P1
200 cycle
Flow Cell
|
n/a
|
n/a
|
NextSeq P2
200 cycle
Flow Cell
|
$2,801
|
$3,922
|
|
|
NextSeq P1
300 cycle
Flow Cell
|
$1,451
|
$2,032
|
NextSeq P2
300 cycle
Flow Cell
|
$3,759
|
$5,262
|
|
|
NextSeq P1
600 cycle
Flow Cell
|
$2,189 | $3,065 |
NextSeq P2
600 cycle
Flow Cell
|
$4,051
|
$5,672
|
|
NovaSeq SP
|
NovaSeq SP
|
||||||
|---|---|---|---|---|---|---|
|
SP Lane ~360 Million Reads
|
SP Flow Cell ~720 Million Reads
|
|||||
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
|
|
NovaSeq SP
100 cycle
Lane
|
$1,424
|
$1,994
|
|
NovaSeq SP
100 cycle
Flow Cell
|
$2,413 |
$3,379
|
|
NovaSeq SP
200 cycle
Lane
|
$1,756
|
$2,458
|
NovaSeq SP
200 cycle
Flow Cell
|
$2,997
|
$4,196
|
|
|
NovaSeq SP
300 cycle
Lane
|
$2,031
|
$2,844
|
NovaSeq SP
300 cycle
Flow Cell
|
$3,628
|
$5,079
|
|
|
NovaSeq SP
500 cycle
Lane
|
$2,497
|
$3,496
|
NovaSeq SP
500 cycle
Flow Cell
|
$4,559
|
$6,382 | |
NovaSeq S4
|
NovaSeq S4
|
||||||
|---|---|---|---|---|---|---|
| S4 Lane ~2250 Million Reads |
|
S4 Flow Cell ~9000 Million Reads | ||||
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
|
|
NovaSeq S4
200 cycle
Lane
|
$3,383 |
$4,737
|
|
NovaSeq S4
200 cycle
Flow Cell
|
$12,539
|
$17,554
|
|
NovaSeq S4
300 cycle
Lane
|
$3,715
|
$5,200
|
NovaSeq S4
300 cycle
Flow Cell
|
$13,863
|
$19,409
|
|
NextSeq 500
|
NextSeq 500
|
||||||
|---|---|---|---|---|---|---|
|
Mid Flow Cell ~100 Million Reads
|
|
High Flow Cell ~300 Million Reads
|
||||
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
|
|
NextSeq Mid
75 cycle
Flow Cell
|
n/a
|
n/a
|
|
NextSeq High
75 cycle
Flow Cell
|
$2,017
|
$2,824
|
|
NextSeq Mid
150 cycle
Flow Cell
|
$1,570
|
$2,197
|
NextSeq High
150 cycle
Flow Cell
|
$3,536
|
$4,951
|
|
|
NextSeq Mid
300 cycle
Flow Cell
|
$2,545
|
$3,563
|
NextSeq High
300 cycle
Flow Cell
|
$5,687
|
$7,962
|
|
NovaSeq S1
|
NovaSeq S1
|
||||||
|---|---|---|---|---|---|---|
|
S1 Lane ~720 Million Reads
|
S1 Flow Cell ~1440 Million Reads
|
|||||
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
|
|
NovaSeq S1
100 cycle
Lane
|
$2,210
|
$3,094
|
|
NovaSeq S1
100 cycle
Flow Cell
|
$3,985
|
$5,578
|
|
NovaSeq S1
200 cycle
Lane
|
$2,659
|
$3,722
|
NovaSeq S1
200 cycle
Flow Cell
|
$4,882
|
$6,835
|
|
|
NovaSeq S1
300 cycle
Lane
|
$3,041
|
$4,258
|
NovaSeq S1
300 cycle
Flow Cell
|
$5,647
|
$7,906
|
|
NovaSeq S2
|
NovaSeq S2
|
||||||
|---|---|---|---|---|---|---|
|
S2 Lane ~1800 Million Reads
|
S2 Flow Cell ~3600 Million Reads
|
|||||
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
Read
Length
|
Harvard
Account
Code
|
PO
Payment
|
|
|
NovaSeq S2
100 cycle
Lane
|
$3,736
|
$5,231
|
|
NovaSeq S2
100 cycle
Flow Cell
|
$7,038
|
$9,853
|
|
NovaSeq S2
200 cycle
Lane
|
$4,522
|
$6,331
|
NovaSeq S2
200 cycle
Flow Cell
|
$8,610
|
$12,053
|
|
|
NovaSeq S2
300 cycle
Lane
|
$4,994
|
$6,992
|
NovaSeq S2
300 cycle
Flow Cell
|
$9,554
|
$13,375 |
|
PacBio Sequel IIe Library Prep & Sequencing
| PacBio Sequel IIe Sequencing | ||
|---|---|---|
|
SMRT Cell ~ 20Gb
~20x coverage for 1 Gb Genome
|
Harvard
Account Code
|
PO
Payment
|
|
SMRT Cell 8M sequencing, per cell
|
$1,838
|
$2,573
|
|
PacBio Library Preparation
(per sample, does not include sequencing)
|
||
|---|---|---|
|
SMRTbell 3.0 Library Prep
|
Harvard Account Code
|
PO Payment
|
|
Whole Genome
(optional barcodes included)
|
$635
|
$898
|
|
IsoSeq
(optional barcodes included)
|
$337
|
$471
|
| 10x MAS-IsoSeq | $1,335 | $1,869 |
|
|
||
|
Recommended QC Services
|
||
|
QC Service, per sample
|
Harvard Account Code
|
PO Payment
|
|
Short Read Eliminator (SRE)
(per sample)
|
$93
|
$130
|
|
Megaruptor Shearing
(per sample)
|
$105
|
$146
|
|
Qubit and Nanodrop
(per sample)
|
$9
|
$13
|
Nanopore Library Prep & Sequencing
|
Oxford Nanopore Technologies (ONT) Sequencing
|
||||||
|---|---|---|---|---|---|---|
| PromethION ~ 80-90 Gb | MinION ~10 Gb | |||||
|
Per Flow Cell
~80-90x coverage
for 1 Gb Genome
|
Harvard
Account Code
|
PO
Payment
|
Per Flow Cell
~1x coverage
for 1 Gb Genome
|
Harvard
Account Code
|
PO
Payment
|
|
|
PromethION Sequencing
|
$1,300
|
$1,820
|
MinION Sequencing | $950 | $1,330 | |
|
Oxford Nanopore Library Prep
(per sample, does not include sequencing)
|
||
|---|---|---|
|
ONT Library Prep Kit
|
Harvard Account Code
|
PO Payment
|
|
Ligation Library
(LSK114)
|
$478
|
$669
|
|
Ligation Library with Native Barcodes
(SQK-NBD114.24)
|
$205
|
$287
|
|
10x Library
(SQK-PCS111)
|
$718
|
$1,006
|
|
Direct RNA Library Preparation
(RNA002)
|
$685
|
$958
|
|
PCR cDNA Library
(SQK-PCS111)
|
$809
|
$1,132
|
|
Barcoded PCR cDNA Library
(SQK-PCB111.24)
|
$273 | $382 |
|
Rapid Library
(SQK-RAD004)
|
$190
|
$266
|
|
Recommended QC Services
|
||
|
QC Service, per sample
|
Harvard Account Code
|
PO Payment
|
|
Qubit and Nanodrop
(per sample)
|
$9
|
$13
|
|
Covaris g-tube Shearing Service
(per sample)
|
$67
|
$94
|
|
Nuclease Flush + ReLoad
|
$150
|
$210
|
Instrument Training
|
Instrument
|
Harvard
Account Code
|
PO
Payment
|
|---|---|---|
|
Sample QC - Purity, Quantification, and Fragment Analysis
|
||
|
Agilent TapeStation
(reagents extra)
|
$170
|
$238
|
|
Spectrophotometers
(Nanodrop & DS-11)
|
No Charge
|
No Charge
|
|
Qubit Fluorometer
|
No Charge
|
No Charge
|
|
Agilent Bioanalyzer
(reagents extra)
|
$340
|
$476 |
|
Shearing & Size Selection
|
||
|
Covaris
|
$128
|
$179 |
|
Pippin Prep/Blue Pippin
|
$128
|
$179
|
|
Sample Preparation & Robotics
|
||
|
Bio-Rad Thermalcyclers
96 & 384 blocks available
|
No Charge | No Charge |
|
10x Genomics Chromium
GEM Formation w/ reagents
(required for independent use)
|
$430/attendee
|
$602/attendee
|
|
Biomek
|
$510
|
$714
|
| Mantis | $276 | $386 |
|
Nanostring nCounter SPRINT
(up to 12 samples)
|
$414
|
$580
|
|
Autogen
|
$415
|
$581
|
|
Real-Time qPCR
|
||
|
BioRad CFX
96 & 384 blocks available
|
$175
|
$245
|
| QX200 droplet digital PCR | $291 | $407 |
|
QX200 ddPCR -additional trainee
|
$39
|
$54
|
|
Imaging & Plate Readers
|
||
|
Azure Sapphire
|
$135
|
$189
|
|
Plate Readers
Neo2, Epoch, Spectramax i3, FlexStation 3
|
No Charge
|
No Charge
|
Instrument Usage
|
Instrument
|
Harvard
Account Code
|
PO
Payment
|
|---|---|---|
|
Sample QC - Purity, Quantification, and Fragment Analysis
|
||
|
Agilent TapeStation
Use fee is included in Tape purchase
See QC & Reagents Fees page
|
$69-$97/tape
|
$95-$136/tape
|
|
Spectrophotometers -Nanodrop & DS-11
|
No Charge
|
No Charge
|
|
Qubit Fluorometer
|
No Charge
|
No Charge
|
|
Agilent Bioanalyzer Self-Run, any assay
|
$13/chip
|
$19/chip
|
|
Shearing & Size Selection
|
||
|
Covaris (includes tube)
|
$7/sample
|
$10/sample
|
|
Pippin Prep/Blue Pippin
|
$17/hr
|
$24/hr
|
|
Sample Preparation & Robotics
|
||
|
Bio-Rad Thermalcyclers
|
No Charge
|
No Charge
|
|
10x Genomics Chromium Controller, any assay
|
$20/chip
|
$28/chip
|
|
Biomek
|
No Charge
|
No Charge
|
|
Mantis
|
$21/hr
|
$29/hr
|
|
NanoString
|
$159/chip
|
$223/chip
|
|
Autogen
|
$15/hr
|
$21/hr
|
|
Real-Time qPCR
|
||
|
BioRad CFX
|
$2/30 minutes
|
$3/30 minutes
|
|
QX200 Droplet Generator -per cartridge
|
$24/ 8-samples
|
$34/ 8-samples
|
|
QX200 Droplet Reader -per cartridge
|
$14/ 8 samples
|
$20/ 8 samples
|
|
Imaging & Plate Readers
|
||
|
Azure Sapphire
|
$7/15 minutes
|
$10/15 minutes
|
|
Plate Reader: Neo2
|
$2/hr
|
$2/hr
|
|
Plate Reader: Epoch
|
$1/hr
|
$2/hr
|
|
Plate Reader: Spectramax i3
|
$4/30 minutes
|
$6/30 minutes
|
|
Plate Reader: FlexStation 3
|
$10/30 minutes
|
$14/30 minutes
|