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Library Prep & QC

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The Bauer Core supports a variety of DNA and RNA library preparation depending on your sample type, concentration, volume, etc. Quality checks before and after the library preparation allow us to guarantee the output of samples that fall within specified...

FAQ

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Our FAQs are updated regularly. Select a main topic below to start. If you do not find your question or still have questions, you can always reach out to Core Staff. Resources Card Access - NWL Building & Core Instrument Login, Scheduling, and Network...

Chiara Gerhardinger

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Chiara is an expert in our single-cell methods. She is a go-to for consultations regarding RNA extractions and is responsible for Illumina sequencing, library preparation, and QC.

Kristina Belanger

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Kristina is responsible for Illumina sequencing, library preparation, training, and QC. She has a focus in lab automation and single-cell library preparation.

Kelly Cribari

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Kelly is responsible for long-read sequencing, library preparation, training, and QC. She supports the flow cytometry core and training on the Nanostring nCounter SPRINT.

Christian Daly

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Christian is responsible for Illumina sequencing. He also trains and supports researchers on the Biomek liquid handlers and plate readers.

Maria E. Lopez-Siegel

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Maria manages the billing for all Division of Science service centers including all the core facilities, the Office of Animal Resources, Research Computing, the Helium Recovery Facility, and the Center for Biological Imaging.

Jeffery Nelson

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Jeff leads the flow cytometry team and is responsible for training and support on flow cytometry sorters, analyzers, and plate readers.

Sarah Lyn Elwell

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Sarah oversees many areas of science operations including core facilities.

Acknowledging the Bauer Core

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We request that you acknowledge the Core when publishing work done with our support. Please refer to us as " The Bauer Core Facility at Harvard University."

QC and Other Services

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The Bauer Core offers a variety of services to assess the quality of your samples and prepare samples for downstream services or methods. Determining the initial quality of your samples is an important step to ensure that sequencing results are accurate...

Next-Generation Sequencing

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Next-Generation Sequencing (NGS) on Illumina instruments allows for massively parallel high-throughput sequencing. Multiple samples can be indexed (barcoded) and combined to make the most of the system's output, resulting in highly accurate and cost...